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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1974/75
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976
Report date:
1976

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Principles of method if other than guideline:
BASF-Test
GLP compliance:
no
Test type:
other: BASF method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Anthra[9,1,2-cde]benzo[rst]pentaphene-5,10-dione, bromo derivatives
EC Number:
215-516-3
EC Name:
Anthra[9,1,2-cde]benzo[rst]pentaphene-5,10-dione, bromo derivatives
Cas Number:
1328-18-3
Molecular formula:
C34H14Br2O2
IUPAC Name:
anthra[9,1,2-cde]benzo[rst]pentaphene-5,10-dione, bromo derivatives
Test material form:
solid
Details on test material:
Vat Blue 19

Test animals

Species:
rat
Strain:
not specified
Sex:
not specified

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
CMC (carboxymethyl cellulose)
Remarks:
0.5% aqueous solution
Details on oral exposure:
Concentration in vehicle: 1.5 to 35%
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: yes
Statistics:
NA

Results and discussion

Preliminary study:
NA
Effect levelsopen allclose all
Sex:
not specified
Dose descriptor:
LD50
Effect level:
ca. 10 000 mg/kg bw
Based on:
test mat.
Key result
Sex:
not specified
Dose descriptor:
LD50
Effect level:
ca. 4 000 mg/kg bw
Based on:
act. ingr.
Mortality:
No data
Clinical signs:
slight apathy, diarrhoea, discoloration of urine and feces, dyspnea
Body weight:
No data
Gross pathology:
heart: acute dilatation, acute hyperemia
stomack: dilated, fluidly content
intestines: diarrheal content
muscles and organs: slight discoloration

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
In an acute oral toxicity study, the LD50 of the test item was determined to be ca. 4000 mg/kg bw (act. ingr.) in rats.
Executive summary:

In an acute oral toxicity study, rats were given a single oral dose of 1.5 - 35 % suspension of the test item diluted in 0.5% aqueous carboxymethylcellulose and were observed for 7 days. Adverse clinical signs of toxicity were observed, such as slight apathy, diarrhoea, dark blue discoloration of urine and feces, and dyspnea. Moreover, gross pathological findings were acute hyperemia and cardiac dilatation, dilated and fluidly content of the stomach, diarrheal content in the intestines and slight discouluration of the muscles and organs. Based on the results from this study, the LD50 of the test item was determined to be approximately 10 000 mg/kg in rats (test. mat.) corresponding to ca. 4000 mg/kg bw (act. ingr.).