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EC number: 285-749-3 | CAS number: 85136-25-0
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- yes
- Test organisms (species):
- Lemna minor
- Details on test organisms:
- - Source : Federal Enviromental Agency (Berlín, Alemania)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Test temperature:
- 24±2ºC
- pH:
- 5.38-6.50
- Nominal and measured concentrations:
- Concentrations tested (active material): 0.85 mg/L, 2.73 mg/L, 8.54 mg/L, 27.32 mg/L, 85.38 mg/L, 273.2 mg/L and 853.8 mg/L
Actual concentration of the test stock solution (active material): 855.5 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 150 ml beaker
- Material, size, headspace, fill volume: glass with 100 ml of test medium
GROWTH MEDIUM
- Standard medium used: yes
Test medium with the following composition:
Macroelements
— Solution 1: KNO3 17,5 g/L H2O; KH2PO4 4,5 g/L H2O; K2HPO4 0,63 g/L H2O
— Solution 2: MgSO4 7H2O 5 g/L H2O
— Solution 3: Ca(NO3)2 4H2O 14,75 g/L H2O
Microelements
— Solution 4: H3BO3 120mg/L H2O
— Solution 5: ZnSO4 7H2O 180 mg/L H2O
— Solution 6: Na2MoO4 2H2O 44,0 mg/L H2O
— Solution 7: MnCl2 4H2O 180,0 mg/L H2O
— Solution 8: FeCl3 6H2O 760,0 mg/L H2O
AEDT Na2 2H2O 1500,0 mg/L H2O
Add to 1000 mL of deionized sterile water:
Solution 1 20 mL
Solution 2 20 mL
Solution 3 20 mL
Solution 4 1 mL
Solution 5 1 mL
Solution 6 1 mL
Solution 7 1 mL
Solution 8 1 mL
ILLUMINATION:
Intensity and Quality of light: 6500 Lux
The surface where the test is performed receives uniform fluorescent lighting and continuous (white-cold type). - Reference substance (positive control):
- yes
- Remarks:
- 3,5-diclofenol
- Key result
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 1 195 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 60.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Results with reference substance (positive control):
- The validation criterion considered for this study is:
- the average number of fronds in the control must present a growth rate specific medium of at least 0.275 per day.
- Duplication time of approximately 2.5 days, and multiplication by 7 of the number of fronds, at the end of the study
- The EC50 7d value of 3,5-diclofenol is in the range 2.2 - 3.8 mg / L. - Validity criteria fulfilled:
- yes
- Conclusions:
- The Direct Blue 281 test product (PF12016 G-D) has a value of EC50 (7d ) of inhibition of the growth rate equal to 1195 mg / L and an inhibition value of biomass EC50 (7d) equal to 60.2 mg/L of active material in Lemna minor.
- Executive summary:
The study of acute toxicity to aquatic plants of Direct Blue dye 281was carried out.
For the determination of the inhibition of algal growth, the freshwater aquatic plant Lemna minor has been used and the operative method has been followed according to the OECD Guideline 221 (2006).
The freshwater aquatic plant Lemna minor has been subject to the substance at the test concentrations: 0.85 mg/L, 2.73 mg/L, 8.54 mg/L, 27.32 mg/L, 85.38 mg/L, 273.2 mg/L and 853.8 mg/L. The total duration of the trial has been 7 days.
The result obtained in this test indicates that the tested substance has an inhibition value of the growth rate EC50 (7d) equal to 1195 mg / L and an inhibition value of biomass EC50 (7d) equal to 60.2 mg / L of active material after 7 days of testing.
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- yes
- Test organisms (species):
- Lemna minor
- Details on test organisms:
- - Source : Federal Enviromental Agency (Berlín, Alemania)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Test temperature:
- 24±2ºC
- pH:
- 5.38-6.50
- Nominal and measured concentrations:
- Concentrations tested (active material): 0,92 mg/L, 2,9 mg/L, 9,2 mg/L, 29,3 mg/L, 91,6 mg/L, 293 mg/L y 916 mg/L
Actual concentration of the test stock solution (active material): 918,7 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 150 ml beaker
- Material, size, headspace, fill volume: glass with 100 ml of test medium
GROWTH MEDIUM STEINBERG
- Standard medium used: yes
Test medium with the following composition:
Macroelements
— Solution 1: KNO3 17,5 g/L H2O; KH2PO4 4,5 g/L H2O; K2HPO4 0,63 g/L H2O
— Solution 2: MgSO4 7H2O 5 g/L H2O
— Solution 3: Ca(NO3)2 4H2O 14,75 g/L H2O
Microelements
— Solution 4: H3BO3 120mg/L H2O
— Solution 5: ZnSO4 7H2O 180 mg/L H2O
— Solution 6: Na2MoO4 2H2O 44,0 mg/L H2O
— Solution 7: MnCl2 4H2O 180,0 mg/L H2O
— Solution 8: FeCl3 6H2O 760,0 mg/L H2O
AEDT Na2 2H2O 1500,0 mg/L H2O
Add to 1000 mL of deionized sterile water:
Solution 1 20 mL
Solution 2 20 mL
Solution 3 20 mL
Solution 4 1 mL
Solution 5 1 mL
Solution 6 1 mL
Solution 7 1 mL
Solution 8 1 mL
ILLUMINATION:
Intensity and Quality of light: 6500 Lux
The surface where the test is performed receives uniform fluorescent lighting and continuous (white-cold type). - Reference substance (positive control):
- yes
- Remarks:
- 3,5-diclofenol.
- Key result
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 1 087 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 112 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Results with reference substance (positive control):
- The validation criterion considered for this study is:
- the average number of fronds in the control must present a growth rate specific medium of at least 0.275 per day.
- Duplication time of approximately 2.5 days, and multiplication by 7 of the number of fronds, at the end of the study
- The EC50 7d value of 3,5-diclofenol is in the range 2.2 - 3.8 mg / L, value 2.9 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The Direct Blue 281 test product (PF12016 G-D) has a value of EC50 (7d ) of inhibition of the growth rate equal to 1087 mg / L and an inhibition value of biomass EC50 (7d) equal to 112 mg/L of active material in Lemna minor.
- Executive summary:
The study of acute toxicity in aquatic plants of Direct Blue dye 281was carried out.
For the determination of the inhibition of algal growth, the freshwater aquatic plant Lemna minor has been used and the operative method has been followed according to the OECD Guideline 221 (2006).
The freshwater aquatic plant Lemna minor has been subject to the substance at the test concentrations: 0,92 mg/L, 2,9 mg/L, 9,2 mg/L, 29,3 mg/L, 91,6 mg/L, 293 mg/L y 916 mg/L. The total duration of the trial has been 7 days.
The result obtained in this test indicates that the tested substance has an inhibition value of the growth rate EC50 (7d) equal to 1087 mg / L and an inhibition value of biomass EC50 (7d) equal to 112 mg / L of active material after 7 days of testing.
Referenceopen allclose all
Description of key information
There are two studies conducted on the substance Direct Blue 281 (CAS 85136 -25 -0) according to the OECD Guideline 221 to determine the toxicity to aquatic plants Lemna minor. Based on the ECHA guidances, this test method is considered more suitable
for testing toxicity of coloured test materials than the OECD guideline 201 to test the toxicity to aquatic algae.
The EC50 (7d) values 1192 mg/L active ingr. and 1087 mg/L active ingr. obtained in these two studies, show that the tested substance is not harmful to this type of aquatic plant.Key value for chemical safety assessment
- EC50 for freshwater plants:
- 1 139.5 mg/L
Additional information
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Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
