Potassium isooctadecanoate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Justification for type of information:

The presented study was conducted with isooctadecanoic acid. The read across source substance is considered a structural analogue with regard to environmental fate and effects on aquatic organisms taking into account the physico-chemical properties (surface active properties, logKow, water solubility) and the chemical structure of the test item (potassium soap of isooctadecanoic acid) and therefore the results of the source substance are considered relevant for risk assessment. See read across document for further details.

Qualifier:

according to guideline

Guideline:

other: Bewertung wassergefährdender Stoffe, Bestimmung der akuten Fischtoxozität, Ad-hoc Arbeitsgruppe 1, LTwS, Nr. 10, Sept 1979

Deviations:

yes

Remarks:

Total exposure duration 48 h instead of 96h exposure of a guideline OECD 203 study. Use of Tween 80 as dispersing agent

Principles of method if other than guideline:

- Total exposure duration 48 h instead of 96h exposure of a guideline OECD 203 study.
- Use of Tween 80 as dispersing agent

GLP compliance:

yes

Specific details on test material used for the study:

- Name of test material (as cited in study report): Prisorine 3505, Isostearic acid
- Physical state: liquid
- Analytical purity: +/- 100 %
- Lot/batch No.: 80011
- Storage condition of test material: at ambient temperature in the dark

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

Preparation and application of test solution(especially for difficult test substances):
- Test substance was homogenuously dispersed using Tween 80 (10 mg/L)
- Controls: yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Test organism
- Source: Zodiac, Wageningen, the Netherlands
- Length at study initiation (length definition, mean, range and SD): 2-4 cm
- Feeding during test: no

Acclimatization
- Type and amount of food: Artemia and Trouvite 00
- Feeding frequency: at least once a day
- Health during acclimation (any mortality observed): mortality during seven days prior to start of test was less than 1%

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

11.7°DH (DSW: Dutch Standard Water)

Test temperature:

20 - 21°C

pH:

0 - 56 mg/L: 7.8 - 8.4
100 mg/L: 7.0 - 7.6

Dissolved oxygen:

8.1 - 8.6 mg/L

Nominal and measured concentrations:

Nominal concentrations: 0, 1, 1.8, 3.2, 5.6, 10, 18, 32, 56, 100 mg/L
No actual concentrations measured.

Details on test conditions:

Test system/Test vessel
- Material, size, headspace, fill volume: 10 L glass vessels
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1

Test medium / Water parameter
- Source/preparation of dilution water: Dutch Standard Water (DSW):Ca2+: 54.5 mg/LMg2+: 17.8 mg/LNa+: 27.4 mg/LK+: 7.8 mg/LCl-: 96.3 mg/LHCO3-: 84.8 mg/LSO42-: 70.2 mg/L

Effect parameters measured (with observation intervals if applicable): mortality, after 2-3h, 24h, 48h

Test concentrations
- Range finding study - Test concentrations: 0.01 to 1000 mg/L (factor 10)
- Results used to determine the conditions for the definitive study: yes

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

29.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (total fraction)

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 24.3 - 39.2 mg/L

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

13.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (total fraction)

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 10.9 - 16.5 mg/L

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (total fraction)

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (total fraction)

Basis for effect:

mortality (fish)

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

5.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (total fraction)

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: After 48 hours alive fish at 10 mg/L showed a decrease in swimming ability.
- Mortality of control: none
- Effect concentrations exceeding solubility of substance in test medium: yes, but use of solubiliser Tween 80.

Sublethal observations / clinical signs:

This study determined a LC50 for the test substance of 13.4 mg/L after 48 hours. Thus it can be expected that the LC50 after 96 hours will be lower. The LC50 exceeds the water solubility of the test substance. The homogeous dispersion of the test substance in water could only be reached using the solubiliser Tween 80.

Validity criteria fulfilled:

yes

Conclusions:

The LC50 for the test substance is 13.4 mg/L after 48 hours. The LC50 exceeds the water solubility of the test substance. The homogeous dispersion of the test substance in water could only be reached using the solubiliser Tween 80.

Executive summary:

The LC50 for the test substance is 13.4 mg/L after 48 hours. The LC50 exceeds the water solubility of the test substance. The homogeous dispersion of the test substance in water could only be reached using the solubiliser Tween 80.

Description of key information

The LC50 for the test substance is 13.4 mg/L after 48 hours. The LC50 exceeds the water solubility of the test substance. The homogeous dispersion of the test substance in water could only be reached using the solubiliser Tween 80.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

13.4 mg/L