Ethanethiol

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1977

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: This study is classified as reliable without restriction because it was conducted according to or similar to now existing guidelines.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

Observation period was only 7 days rather than the recommended 14 day duration.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Ethanethiol

EC Number:

200-837-3

EC Name:

Ethanethiol

Cas Number:

75-08-1

Molecular formula:

C2H6S

IUPAC Name:

ethanethiol

Test animals

Species:

rat

Strain:

not specified

Sex:

male

Administration / exposure

Type of coverage:

occlusive

Vehicle:

other: none

Duration of exposure:

24 hours

Doses:

2000 mg/kg

No. of animals per sex per dose:

10

Control animals:

not specified

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Any other information on results incl. tables

Initial skin contact evoked a severe pain reaction which coincided with a slight darkening in the color of the skin, none of the treated animals showed any toxic symptoms and all gained body weight during the subsequent period of observation. None of animals died following the test substance administration

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The acute dermal LD50 was determined to be >2000 mg/kg bw (Latven, 1977).

Executive summary:

In a key acute dermal toxicity study, 10 male albino WBS/W rats were dermally administered ethanethiol (ethyl mercaptan) at doses of 2000 mg/kg bw (Latven, 1977). The test material was applied to clipped trunks under an impervious sleeve for a period of 24 hours and the animals were subsequently observed for 7 days.

There was no mortality observed through the study period but initial skin contact with the test material produced a mild pain reaction that was accompanied by dark discolouration of the skin. There were no other treatment-related effects observed through the study. The acute dermal LD₅₀was determined to be >2000 mg/kg bw.

This study received a Klimisch score of 2 and is classified as reliable with restrictions because it adheres closely to OECD guideline but does not follow GLP.