Reaction mass of (2S,6S)-2,6-Dimethylmorpholine and (2R,6R)-2,6-Dimethylmorpholine

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented study report which meets basic scientific principles

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Principles of method if other than guideline:

BASF-Test, see details in remarks on material and methods.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Automatically generated during migration to IUCLID 6, no data available

IUPAC Name:

Automatically generated during migration to IUCLID 6, no data available

Details on test material:

- Name of test material (as cited in study report): 2,6-dimethyl-morpholin (75 % cis / 25 % trans)
- Physical state: liquid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: males: 266 g (mean), females: 200 g (mean)
- Fasting period before study: 16-20 h
- Diet: Herlian MRH-Haltung, ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 6.81, 10.0, 14.7, 21.5, 31.60 %

Doses:

681, 1000, 1470, 2150, 3160 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: several times on the day of application and daily thereafter
- Frequency of weighing: days 0, 2-4, 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1 270 mg/kg bw

95% CL:

1 035 - 1 551

Mortality:

See details in table in remarks on results.

Clinical signs:

Dyspnoea, apathy, staggering, atony, tremor, spastic gait, convulsions, ruffled fur, cyanosis, exsiccosis, salivation, poor general state

Body weight:

initially weight loss; see details in table in remarks on results.

Gross pathology:

Heart: dilatation (right), congestion hyperemia;
Stomach: strong, diffuse redding, bloody content;
Gut: dark-diffuse redding of mucosa, diarrhoea;

Any other information on results incl. tables

Mortality:

Dose mg/kg bw	Conc. %	Dead animals
3160	31.6	5/5 m, 5/5 f
2150	21.5	5/5 m, 3/5 f
1470	14.7	4/5 m, 4/5 f
1000	10	1/5 m, 2/5 f
681	6.81	0/5 m, 0/5 f

Weight:

Dose (mg/kg bw)	gender	day 0	day 2-4	day 7	day 14
3160	m	290	-	-	-
3160	f	210	-	-	-
2150	m	240	-	-	-
2150	f	180	184	207	223
1470	m	250	235	277	312
1470	f	200	193	215	232
1000	m	260	244	295	328
1000	f	200	199	214	231
681	m	290	309	364	348
681	f	210	224	269	242