Registration Dossier
Registration Dossier
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EC number: 212-028-2 | CAS number: 751-83-7
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Test procedure according to OECD guidlines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- [3-methyl-5-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2,4-pentadienyl]triphenylphosphonium sulphate
- EC Number:
- 212-028-2
- EC Name:
- [3-methyl-5-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2,4-pentadienyl]triphenylphosphonium sulphate
- Cas Number:
- 751-83-7
- Molecular formula:
- C33H38P.HO4S
- IUPAC Name:
- mono[3-methyl-5-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2,4-pentadienyl]triphenylphosphonium hydrogensulphate
- Details on test material:
- - Name of test material (as cited in study report): C15-Salz kristallin ((3-Methyl-5-(2,6, 6-trimethyl- 1 -cyclohexen- 1-yl)-2,4- pentadienyl)triphenylphosphoniumsulfat)
- Purity: ca. 90 %
- Batch No.: 96/2-315 + 316
- Solubility in water: 28.0 g/L at 20 °C
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- - Animal Species: Zebra fish (Brachydanio rerio KAM. and BUCH.)
- Animal breeder/supplier: Bio International B.V., NJ Horn, Netherlands Hatching date: Sep. 14, 1997
- Age of the animals: About 4 months
- Body length: 3.25 (2.9 - 3.8) cm
- Body weight: 0.21 (0.13 - 0.37) g
- Corpulence factor of the batch: 0.6
- Diet: 'Tetra min', standard feed for aquarium fish
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- ca. 2.5 mmol/L
- Nominal and measured concentrations:
- Nominal test concentration: 0, 0.0464, 0.1, 0.215, 0.464, 1.0 mg/L
Analytically detected concentrations (study initiation):in the range of 0 - 10.0 % of the nominal concentration
Analytically detected concentrations (after 96 hours): in the range of 10.0 % - 19.0 % of the nominal concentration - Details on test conditions:
- TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours darkness
- Volume of water: 10 L (water depth about 17 cm).
- No. of animals per vessel: 10
- Loading (g fish/l water): 0.2 g/L
- Test temperature: 22 °C
- Withdrawal of food: 1 day before and during exposure
- Aeration: None
TEST MEDIUM / WATER PARAMETERS
- Test water: Municipal water of the City of Frankenthal, not chlorinated and passed through a charcoal filter, aerated.
- Total hardness: approx. 2.5 mmol/L (= approx. 250 mg/l CaCO3)
- Acid capacity: approx. 5.5 mmol/L
- Ca content: approx. 90 mg/L
- Mg content: approx. 10 mg/L
- pH: approx. 8.0 - 8.6
TEST CONCENTRATIONS
- Range finding study: Based on the results of 2 range finding studies (static system), the LC50 after 96 h was found between 0.1 and 1.0 mg/L.
- Spacing factor for test concentrations: 2.2
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.06 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 0.19 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.03 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
- Results on the basis of the nominal concentrations
LC0 = 0.215 mg/lLC50 = ca. 0.5343 mg/l (value of interpolation
LC100 = 1.0 mg/lNOEC = 0.215 mg/l
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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