Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex

EC Number:

413-650-9

EC Name:

Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex

Cas Number:

93971-95-0

Molecular formula:

Can not be identified

IUPAC Name:

hexacopper(2+) 5-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,14-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,15-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 7-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 8-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

-Type : A mixed population of activated sewage sludge micro-organisms.
-Source : The aeration stage of the Severn Trent Plc sewage treatment plant at Belper, Derbyshire, treating predominantly domestic sewage.
-Preparation : The sample was maintained at 21ºC with continuous aeration and used on the day of collection.
The pH was equal to 6.3 and the suspended solids level was equal to 3.9 g/l.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

3 h

Test conditions

Hardness:

approximately 50 mg/l as CaCO3

Test temperature:

21ºC

pH:

6.3

Nominal and measured concentrations:

Test material : 1000 mg/L in triplicate.
Reference material : 3.2, 10 and 32 mg/L.

Details on test conditions:

Dilution Water
-Type : Laboratory tap water, dechlorinated by sodium thiosulphate.
Synthetic Sewage :
-Type : 16g peptone
11 g meat extract
3 g urea
0.7 g NaCl
0.4 g CaCl2.2H2O
0.2 g MgSO4.7H2O
2.8 g K2HPO4
- dissolved in 1 litre tap water.
Exposure Conditions
-Contact time : 30 minutes and 3 hours.
-Vessels : 500 ml conical flasks.
-Aeration : Compressed air via narrow bore glass tubes.
-Measuring apparatus : 250 ml darkened glass BOD bottle fitted with a Yellow Springs BOD probe, (Model 54) and a magnetic stirrer.
-Photoperiod : Normal laboratory lighting.
-Control : Inoculated medium without test or reference material.
-Procedure : At time "0" 16 ml of synthetic sewage was made up to 300 ml with water in a 500 ml conical flask. 200 ml inoculum were added and the mixture aerated at approximately 0.5-1 litre per minute. Thereafter, at 15 minute intervals the procedure was repeated with appropriate amounts of the test or reference material being added.
As each vessel reached 30 minutes contact time the contents were poured into the measuring vessel and the rate of respiration measured. The procedure was repeated after a 3-hour contact period.

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Reported statistics and error estimates:

30 minutes
Test material EC50 : >1000 mg/L
Reference material EC50 : 16.5 mg/L
Varidation in respiration rates of Controls 1 and 2 : +-2%

3 hours
Test material EC50 : >1000 mg/L
Reference material EC50 : 11.5 mg/L
Varidation in respiration rates of Controls 1 and 2 : Zero

It was considered unnecessary and unrealistic to test at concentrations in excess of 1000 mg/L.

Any other information on results incl. tables

Oxygen consumption Rates and Percentage Inhibition for 30 Minutes Contact Time  Material     Concentration  O2 consumption rates  inhibition  (mg/l)  mg O2/l/min  %  Control     R1 0.53  -  R2 0.51  -  Test material        1000 R1 0.50  4  1000 R2 0.53  <2>  1000 R3 0.52  0  Reference material        3.2 0.48  8  10 0.43 17  32 0.14 73           <increase>           R1, R2 and R3 Replicates 1, 2 and 3

Oxygen consumption Rates and Percentage Inhibition for 3 Hours Contact Time Material  Concentration  O2 consumption  inhibition  (mg/l)  mg O2/l/min  % Control  R1 0.52 -  R2 0.52 - Test material         1000 R1 0.51 2  1000 R2 0.52 0  1000 R3 0.51 2 Reference material  3.2 0.49 6  10 0.29 44  32  0.07 87           R1, R2 and R3 Replicates 1, 2 and 3