Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex

EC Number:

413-650-9

EC Name:

Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex

Cas Number:

93971-95-0

Molecular formula:

Can not be identified

IUPAC Name:

hexacopper(2+) 5-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,14-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,15-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 7-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 8-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

Direct dispersion in water with the aid of shielded propeller-stirres. 2.0 g of test material were dispersed in 20 litters of water with the aid of shielded propeller-stirreres to give a 100 mg/l test concentration. 1% Tween 80-dimethylsulphoxide at a concentration of 100 μl/l was added to aid dispersal of the test material.

Test organisms

Test organisms (species):

other: RAINBOW TROUT

Details on test organisms:

-Souce : Parkwood Trout Farm, Wigmore, Kent, U.K.
-Mean standard length : 5.3 cm (SD = 0.7 cm)
-Mean weight : 1.57g (SD = 0.45 g)

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

96 h

Test conditions

Hardness:

approximately 50 mg/L as CaCO3

Test temperature:

14 °C

pH:

7.5 - 7.6

Dissolved oxygen:

10.0 -10.1 mg O2/L

Nominal and measured concentrations:

Nominal concentration : 100 mg/L
Concentration found 0 hour : 94.3 mg/L, 96.7 mg/L
Concentration found 24 hours : 103 mg/L, 94.4 mg/L
Concentration found 96 hours : 89.6 mg/L, 92.1 mg/L

Details on test conditions:

Test water : Laboratory tap water, dechlorinated by the addition of sodium thiosulphate.
Test vessels : Glass aquaria holding 20 l of test media.
Experimental design : 1 test concentration in duplicate plus 1 control and 1 solvent control (including 100 μl/l of auxiliary solvent). 20 animals per test concentration.
Method of initiation : Fish were placed, at random, in prepared test media.
Loading : 0.79 g bodyweight/litre.
Photoperiod : 16h light, 8h dark.
Aeration : By shielded propeller-stirrers.
Test concentration : 100 mg/l, in duplicate.
Media renewal : Daily.
Criteria of death : Absence of (i) respiratory movement and (ii) response to a physical stimulation.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Cumulative Mortality Date for Rainbow Trout Expose for 96 hours.  Concentration  Cumulative mortality (initial population : 10)                 mg/l  3h  6h  24h  48h  72h  96h  Control  0  0  0  0  0  0  Solvent control  0  0  0  0  0  0  100 R1  0  0  0  0  0  0  100 R2  0  0  0  0  0  0              R1, R2 = Replicates R1 and R2

Applicant's summary and conclusion

Conclusions:

LC50 > 100mg/L
No Observed Effect Concentration(NOEC) ≧100mg/L