Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 929-305-5 | CAS number: -
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Performed to GLP meeting current test methods Quality criteria fulfilled
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- Salmonella typhimurium strains TA1535, TA1537, TA98 and TA100 and Escherichia coli strain WP2uvrA were treated with the test item using both the Ames plate incorporation and pre-incubation methods at up to eight dose levels, in triplicate, both with and without the addition of S9
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- (2-aminoethyl)(2-{[(2E)-3-methoxy-2-methyl-3-(octylsulfanyl)prop-2-en-1-yl]amino}ethyl)amine; 11-methoxy-12-methyl-3-(prop-1-en-2-yl)-2-oxa-4,7,10,14,17-pentaazanonadecan-19-amine; 2-methyl-3-(octylsulfanyl)-N-{2-[(2-{2-[(octylsulfanyl)methyl]propanamido}ethyl)amino]ethyl}propanamide; 3-({2-[(2-aminoethyl)amino]ethyl}amino)-2-methyl-N-{2-[(2-{2-[(octylsulfanyl)methyl]propanamido}ethyl)amino]ethyl}propanamide; N-{2-[(2-aminoethyl)amino]ethyl}-2-methyl-3-(octylsulfanyl)propanamide
- EC Number:
- 929-305-5
- IUPAC Name:
- (2-aminoethyl)(2-{[(2E)-3-methoxy-2-methyl-3-(octylsulfanyl)prop-2-en-1-yl]amino}ethyl)amine; 11-methoxy-12-methyl-3-(prop-1-en-2-yl)-2-oxa-4,7,10,14,17-pentaazanonadecan-19-amine; 2-methyl-3-(octylsulfanyl)-N-{2-[(2-{2-[(octylsulfanyl)methyl]propanamido}ethyl)amino]ethyl}propanamide; 3-({2-[(2-aminoethyl)amino]ethyl}amino)-2-methyl-N-{2-[(2-{2-[(octylsulfanyl)methyl]propanamido}ethyl)amino]ethyl}propanamide; N-{2-[(2-aminoethyl)amino]ethyl}-2-methyl-3-(octylsulfanyl)propanamide
- Test material form:
- liquid: viscous
- Details on test material:
- Batch 5040-57
Stated purity > 99%
Amber viscous liquid
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat liver S-9
- Test concentrations with justification for top dose:
- Eight concentrations of the test item in Experiment 1
1.5, 5, 15, 50, 150, 500, 1500 and 5000 ug/plate
The dose range used for Experiment 2 was determined by the results of Experiment 1 and was :
All Salmonella strains (absence of S9-mix): 0.15, 0.5, 1.5, 5, 15, 50, 150 μg/plate.
All Salmonella strains (presence of S9-mix): 0.5, 1.5, 5, 15, 50, 150, 500 μg/plate.
E.coli strain WP2uvrA (absence and presence of S9-mix): 1.5, 5, 15, 50, 150, 500, 1500 μg/plate. - Vehicle / solvent:
- Sterile distilled water
- Details on test system and experimental conditions:
- The S9 Microsomal fraction was prepared in-house from male rats induced with Phenobarbitone/-Naphthoflavone
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Toxicity noted at highest treatment levels, typically above 1500 ug/plate.
No differences in toxicity between replicates with or without S-9 - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Negative with and without S-9
Toxic at treatment levels of 1500 ug/plate and higher.
No significant differences in cytotoxicty between replicates with or without S-9
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
