Isotridecanol, ethoxylated

Toxicity to terrestrial plants

Link to relevant study record(s)

Reference

Endpoint:

toxicity to terrestrial plants: short-term

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

First test: 18 Apr - 05 Mai 1994, Second test 18. Juli - 04 Aug 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Please refer to the Analogue Approach Justification provided in Section 13.

Reason / purpose for cross-reference:

read-across: supporting information

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil (if used):
first test: 0.16 g auf 20 g quarz sand + 1580 g substrate
second test: 0.001 g auf 20 g quarz sand + 980 g substrate (= 1mg/kg), - 0.006 g auf 20 g quarz sand + 580 g substrate (= 10 mg/kg), - 0.06 g auf 20 g quarz sand + 580 g substrate (= 100 mg/kg)

Species:

Triticum aestivum

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: wheat
- Plant family: Poaceae

Species:

Lepidum sativum

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: garden cress
- Plant family: Brassicaceae

Species:

Brassica alba

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: mustard
- Plant family: Brassicaceae

Test type:

seedling emergence toxicity test

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

17 d

Test temperature:

First test: 22 - 25 °C
Second test: 20 - 25 °C

pH:

5 - 7.5

Moisture:

59 - 65% WHC

Details on test conditions:

TEST SYSTEM
- Test container (type, material, size): plastic, diameter bottom: 6 cm, top: 8 cm, height: 6 cm
- Amount of soil: approximately 100 g
- No. of seeds per container: 5
- No. of replicates per treatment group: 4
- No. of replicates per control: 4


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: LUFA soil Speyer (Sp. 2.3), sieved (0.5 cm)
- Soil texture (if natural soil): particle < 20 µm: 10 - 20%
- Organic carbon (%): < 1.5%



GROWTH CONDITIONS
- Photoperiod: 16 h day / 8 h night
- Light intensity and quality: day: first test 1500 - 2000 lux, night: approximately 50 lux; second test 1000 - 2000 lux, night: approximately 50 - 100 lux
- Day/night temperatures: First test: 22 - 25 °C; Second test: 20 - 25 °C
- Relative humidity (%): 59 - 65 % WHC


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Seedling emergence and growth, measured at test end by weighing.

Nominal and measured concentrations:

first test: limit test with nominal concentration: 100 mg/L
second test (Lepidium sativum, only): nominal: 1, 10 and 100 mg/L

Species:

Triticum aestivum

Duration:

17 d

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Triticum aestivum

Duration:

17 d

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

Brassica alba

Duration:

17 d

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Brassica alba

Duration:

17 d

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

Lepidum sativum

Duration:

17 d

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Lepidum sativum

Duration:

17 d

Dose descriptor:

EC0

Effect conc.:

10 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Table 1: Effect on grwoth (plant parts above ground)

	Test substance concentration [mg/kg soil]
	Triticum aestivum		Brassica alba		Lepidium sativum
	0	100	0	100	0	1	10	100
Mean freshweight [mg]	265	310	189	168	103	103	101	68
Standard Deviation	100	82	46	48	34	26	22	10
Number of Seedlings	20	18	16	18	19	19	20	20
Emergence	100%	90%	80%	90%	95%	95%	100%	100%

Table 2. Validity criteria for OECD 208.

Criterion	Outcome	Criterion fulfilled
Seedling emergence in the controls is ≥ 70%.	Triticum aestivum: 100% Brassica alba: 80% epidum sativum: 95%	yes
No visible phytotoxic effects (e.g. chlorosis, necrosis, wilting, deformatons) in control seedlings and the plants of every particular species exhibit normal variation in growth and morphology.	Not observed	yes
The environmental conditions for a particular species are identical and the growing media contain the same amount of soil matrix, support media or substrate from the same source.	yes	yes

 

Validity criteria fulfilled:

yes

Remarks:

Please refer to table 2 at "Any other information on results incl. tables"

Description of key information

EC0 (17d)>= 10 mg/kg soil dw. for growth of Lepidum sativum

Additional information

There is no study available in which the toxicity of Isotridecanol, ethoxylated, < 2.5 EO to soil macroorganisms was assessed. Therefore, read-across to the structurally and chemically closely related source substance Isotridecanol, ethoxylated 3EO was performed in accordance with Regulation (EC) No 1907/2006 Annex XI, section 1.5 o fulfil the data requirements for this substance. The source substance is characterized by similar chemical structure and is therefore considered as suitable representative for the assessment of the toxicity of the target substance to microorganism. A detailed read-across justification in provided in IUCLID section 13.

The study investigated the effect of the test substance on the emergence and growth of three plant species according to OECD guideline 208 and GLP. The tested plant species were wheat, Triticum aestivum, a monocot and the dicots Brassica alba and Lepidum sativum (white mustard and garden cress). In a first test soil was mixed with a stock solution of the test substance to give a nominal exposure concentration of 100 mg/kg soil dw. The seedling emergence was followed for 17 days. At test termination, the plants were harvested and the growth was determined by measuring the weight of the above ground plant parts. Effects on plant growth were only observed for Lepidum sativum. Therefore a second test was performed investigating the growth of Lepidum sativum at test concentrations of 1, 10 and 100 mg/kg soil. An inhibition of plant growth were observed at 100 mg/kg soil dw. An EC0 (17 d)of10 mg/kg soil dw based on plant growth was recorded.