Sodium 3-(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-10-24 to 1991-11-29

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

Principles of method if other than guideline:

NA

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
NA

Analytical monitoring:

no

Details on sampling:

NA

Vehicle:

no

Details on preparation and application of test substrate:

Artificial soil and application of test substance:
Artificial soil for tests was prepared according to OECD 207 before the study started and stored until use in a closed plastic-container. Ingredients:
-10 % Sphagnum-peat, air-dryed and finely groimd (mesh width 2 mm)
- 20 % Kaolin clay, extra pure
- ca. 0.4% chalk (CaC03) extra pure to adjust pH to 6.0 ± 0.5
- ca. 69.6 % fine quartz-sand (grain size 0.05-0.2 mm)
Ultimately before starting the experiments, the test substance was dissolved in bidistilled water and then added to the artifidal soil (laboratory mbcer).
In course of applicating the test substance, the soil was ventilated and moistened to ca. 35% of dry weight with demineralised (mQ) water. Water content and pH were controlled at start and finish of the study for each test concentration.

Test vials
Test vials were normal bottling jars (11), which were loosely covered by glass-lids to enable exchange of air and to minimize evaporation of the artificial soil.

Test organisms (species):

Eisenia fetida

Details on test organisms:

TEST ORGANISM
- Common name:
- Source: bred by RCC
- Age at test initiation (mean and range, SD): only adult worms (older than two months)
- Weight at test initiation (mean and range, SD): 300-600 mg

ACCLIMATION
- Acclimation period: 24 h

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Post exposure observation period:

NA

Test temperature:

20 +/- 2°C

pH:

pH (start)= 5.5 - 5.6
pH (end) = 5.7 - 5.8

Moisture:

The water capacity of the artificial soil was determined to be 50% of the dry weight at the begin of the study.
The water content was ca. 35 - 36% (dry weight) at start and in a range firom 34% to 36% at the end of the study.

Details on test conditions:

At test start, ten worms were placed on the surface of the artificial soil of each test vial (four replicates per concentration/control). The test vials were exposed in an air conditioned room at 20 ± 2 °C. The continuous illumination was measured to be 370-780 lux during the entire study. The vials were controled at days 7 and 14, respectively.

Nominal and measured concentrations:

10, 31.6, 100, 316.2, 1000 mg/kg

Reference substance (positive control):

yes

Remarks:

chloracetamide

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality at end of exposure period: Nearly no worm during of the entire study in the test vessels with test substance as well as io the water
treated controls. In all test concentrations, the body weights of the surviving worms decreased slightly due to the starvation of the animals within the test period.
- Behavioural abnormalities: No behavioural abnormaUties could be observed.

Results with reference substance (positive control):

The last check (conducted in May 1991) of the whole test system with standard compound (Chloracetamide) resulted in a LC50 (14 days) of 10.92 mg/kg artificial soil (95% confidence range: 9.61 -12.41 mg/kg).

Reported statistics and error estimates:

The LC50 and its 95% confidence limits at days 7 and 14 were tried to estimate by Probit-Analysis or Moving Average Interpolation.

NA

Validity criteria fulfilled:

yes

Conclusions:

The LC50 (7,14 days) of sodium 3(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate was > 1000 mg/kg.

Executive summary:

The toxicity of sodium 3(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate towards Eisenia foetida was tested for a exposure of 14 days

according to OECD 207 guideline. During the entire study, the pH of the articial soil was in a range of 5.5 to 5.8. The water capacity of the artificial soil was determined to be 50 % of the dry weight at the beginn of the study. The water content was ca. 35 - 36 % (dry weight) at start and in a range from 24 % to 36 % at the end of the study.

Nearly no worm died during of the entire study in the test vessels with test substance as well as in the water treated controls. Consequently it was not possible to calculate the LC50 of test item to Eisenia foetida, which is clearly higher than 1000 mg/kg artificial soil.

In all test concentrations, the body weights of the surviving worms decreased slightly due to the starvation of the animals within the test period. No behavioural abnormalities could be observed.

The LC50 (7,14 days) of sodium 3(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate was > 1000 mg/kg.

The results of this study did not reveal any toxic effect of test item to E. foetida at the limit concentration of 1000 mg/kg.

Description of key information

LC50(14d) > 1000 mg/kg dw for mortality of Eisenia foetida (OECD 207)

Additional information

The toxicity of sodium 3(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate towards Eisenia foetida was tested for a exposure of 14 days according to OECD 207 guideline. During the entire study, the pH of the articial soil was in a range of 5.5 to 5.8. The water capacity of the artificial soil was determined to be 50 % of the dry weight at the beginn of the study. The water content was ca. 35 - 36 % (dry weight) at start and in a range from 24 % to 36 % at the end of the study. Nearly no worm died during of the entire study in the test vessels with test substance as well as in the water treated controls. Consequently it was not possible to calculate the LC50 of test item to Eisenia foetida, which is clearly higher than 1000 mg/kg artificial soil. In all test concentrations, the body weights of the surviving worms decreased slightly due to the starvation of the animals within the test period. No behavioural abnormalities could be observed. The LC50 (7,14 days) of sodium 3(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate was > 1000 mg/kg. The results of this study did not reveal any toxic effect of test item to E. foetida at the limit concentration of 1000 mg/kg.