Artemisia pallens, ext.

Boiling point

Link to relevant study record(s)

Reference

Endpoint:

boiling point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09/15 February 2021

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

GLP study carried out according to OECD / EU guidelines.

Qualifier:

according to guideline

Guideline:

EU Method A.2 (Boiling Temperature)

Version / remarks:

2008

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 103 (Boiling Point)

Version / remarks:

1995

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Inspected on 15/16 Nov. 2017, signed on 15 May 2018

Type of method:

differential scanning calorimetry

Specific details on test material used for the study:

Appearance: yellow to red, clear liquid
Storage room temperature: 20 ± 5 °C (293.15 ±5 K)

Key result

Boiling pt.:

ca. 205.26 °C

Atm. press.:

ca. 1 011.9 mBar

Results Pre-Test:

All observations of the Pre-Test for Boiling Point and Safety Test are presented in the following tables:

Table 1 Pre-Test for Boiling Point and Safety Test

Observations	Temperatures in °C
Individual bubble	from 51
Condensate was visible	from 76
More condensate	from 110
Bubbles came faster	from 160
Bubble chain began with boiling delay, fizzle and gas in head space, for safety reasons the heating plate was turned off	210
The test item was visually unchanged	after cooling down

Security test for the DSC instrument:

The crucible was found visually unchanged after reaching a temperature of 200 °C, 300 °C,400 °C and 450 °C. The weight of the crucible was recorded with 45.17 mg (weight loss: 2.18 mg, i.e 89.3% of substance).

Table 2 - Values DSC Test Item Pre-Test

Boiling point	Temperature [°C]	Energy (J/g) / Heat flow (mW)
Begin	156.65	18.52 mW
Onset	205.77	193.41 J/g
Peak max.	277.43	-
End	337.23	17.12 mW

Table 3-Values Observations

	Mass test item	Mass crucible (before measurement):	Mass crucible (after measurement):	Observations
DSC P re-test	2.80 mg	47.34 mg	44.66 mg	After the test the crucible was visually unchanged (weight loss: 2.68 mg, i.e. : 95.7%)

Results - Test Method following DSC (Main-test):

After calibration with indium and zinc the measurement of indium yielded the following values:

Table 4 - Values DSC Indium:

Expected Melting point	Measured Melting point	Expected Energy	Measured Energy	Assessment
156.6 ± 1.0°C	156.83 °C (onset)	28.45 ± 1.0 J/g	27.64 J/g	OK

The ambient atmospheric pressure was recorded to be 1011.9 mbar.

Table 5 - Values DSC Test Item first determination

Boiling point	Temperature [°C]	Energy (J/g) / Heat flow (mW)
Begin	162.15	18.75 mW
Onset	205.12	158.28 J/g
Peak max.	284.26	-
End	340.16	16.84 mW

The ambient atmospheric pressure was recorded to be 1011.9 mbar

Table 6 - Values DSC Test Item second determination

Boiling point	Temperature [°C]	Energy (J/g) / Heat flow (mW)
Begin	162.15	18.88 mW
Onset	205.39	156.51 J/g
Peak max.	284.4	-
End	340.14	16.87 mW

Table 7 - Values Observations

	Mass test item	Mass crucible (before measurement):	Mass crucible (after measurement):	Observations
First Measurement	4.15 mg	49.80 mg	45.81 mg	After the test the crucible was visually unchanged (weight loss: 3.99 mg, i.e. : 96.1%)
Second Measurement	4.18 mg	49.41 mg	45.35 mg	After the test the crucible was visually unchanged (weight loss: 4.06 mg, i.e. : 97.1%)

Table 8 - Presentation of Results:

All results are presented in the following table.

	Boiling point	Temperature [°C]	Temperature [K]
1st  Determination	Begin	162.15	435.30
2nd Determination	Begin	162.15	435.30
	Mean	162.15	435.30
	Sd	0.00	0.00
1st  Determination	End	340.16	613.31
2nd Determination	End	340.14	613.29
	Mean	340.15	613.30
	Sd	0.01	0.01
1st  Determination	Onset	205.12	478.27
2nd Determination	Onset	205.39	478.54
	Mean	205.26	478.41
	Sd	0.19	0.19
1st  Determination	Peak max.	284.26	557.41
2nd Determination	Peak max.	284.40	557.55
	Mean	284.33	557.48
	Sd	0.10	0.10

 

 

Conclusions:

Under the test conditions, at the pressure of 1011.9 mBar, the mean onset for boiling occurred at 205.26°C (478.41 K).

Executive summary:

The boiling point of the test substance was determined under GLP according to OECD 103 / EU A.2 guideline, using differential scanning calorimetry (DSC) method.
Two main runs were performed under inert gas atmosphere at the pressure of 1011.9 mBar, and the mean temperature was retained.
In all runs one event was detected. The event is endotherm and is stated as the boiling point.
Under the test conditions, at the pressure of 1011.9 mBar, the mean onset for boiling occurred at 205.26°C (478.41 K).

 

Description of key information

Under the test conditions, at the pressure of 1011.9 mBar, the mean onset for boiling occurred at 205.26°C (478.41 K).

 

Additional information

A fully reliable experimental study, conducted according to a recognized OECD 103 / EU A.2 method and under GLP, is available.
It is considered as a key study, and the result is retained as key data.