1,2,4-triazole-3-thiol

Endpoint summary

Description of key information

Skin irritation:

3-Mercapto-1 , 2 , 4 , -triazol was tested for its primarily skin-irritating properties on rabbits. 0.5 g each was applied to the back skin of 3 test animals. The test product did not cause any skin reactions. The average stimulus index is: 0.0

Eye irritation:

The substance was tested for its eye-irritating properties on rabbits. 0.1 g was placed in the conjunctival sac of 3 experimental animals. Observed changes: Cornea: Slight opacity or turbidity, in which details of the iris were slightly shadowed, affected the entire corneal area. Iris: moderate circulatory corneal hyperemia. Conjunctiva: diffuse flesh-colored redness; clear Swelling, which led to the partial turning of the lids or to the half-closed lids. Other eye changes: Hypersecretion, which moistened the surrounding eyelids and hair or considerable parts around the eye. These findings were no longer than 2 weeks p.appl.demonstrated. The determined stimulus index 40. For 3-mercapto-1, 2, 4-triazole, this results in moderately irritating properties in the rabbit eye. Systemic-toxic effects were not detectable.

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records

Reference

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Remarks:

The stimulus index on the intact skin is determined in a modified form according to Gilman et al (5).

GLP compliance:

not specified

Species:

rabbit

Strain:

Himalayan

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

water

Controls:

yes, concurrent no treatment

Amount / concentration applied:

0.5 g test substance in 0.25 ml aqua dest.
The application site was covered with a roughly 6.25 large linen cloth that adheres to a synthetic adhesive film. An occlusion dressing applied in a circular manner around the trunk (Acrylastic, P. 2408, width 8 on) served to seal off the outside.

Duration of treatment / exposure:

4 hour (occlusive patch)

Observation period:

3 days p. appl.

Number of animals:

3 (two male, one female)

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0

Max. score:

4

Irritation parameter:

erythema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0

Max. score:

4

Irritation parameter:

erythema score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0

Max. score:

4

Irritation parameter:

edema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0

Max. score:

4

Irritation parameter:

edema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0

Max. score:

4

Irritation parameter:

edema score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0

Max. score:

4

Interpretation of results:

GHS criteria not met

Conclusions:

A single application of 0.5 g 3-Mercapto-1,2,4,-triazol each on the intact skin of 3 rabbits caused no changes.
The irritation index is 0.0. 3-Mercapto-1,2,4,-triazol has no irritating properties on the skin of the rabbit.
Systemic-toxic effects did not occur after a single application of the test product. The general condition of the experimental animals was undisturbed.

Executive summary:

3-Mercapto-1,2,4,-triazol was tested for its primarily skin-irritating properties on rabbits. 0.5 g each was applied to the back skin of 3 test animals. The test product did not cause any skin reactions. The average stimulus index is: 0.0.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Eye irritation

Link to relevant study records

Reference

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

not specified

GLP compliance:

not specified

Species:

rabbit

Strain:

Himalayan

Vehicle:

unchanged (no vehicle)

Controls:

yes, concurrent no treatment

Amount / concentration applied:

0,1g

Duration of treatment / exposure:

one time application

Observation period (in vivo):

13 days a.appl.

Number of animals or in vitro replicates:

1 (one male, two females)

Details on study design:

0.1 g of the test substance was placed in the conjunctival sac of the right eye.Then both lids were kept closed for a short time by light finger pressure.The left eye of each animal was left untreated and served as a control. The treated eye was not rinsed.
obersvation tome point: 1, 24, 72 hours, after that ones daily till day 13.

Irritation parameter:

iris score

Basis:

animal #1

Time point:

24 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 2d

Irritation parameter:

iris score

Basis:

animal #2

Time point:

24/48/72 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 4d

Irritation parameter:

iris score

Basis:

animal #3

Time point:

24/48 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 3d

Irritation parameter:

cornea opacity score

Basis:

animal #1

Time point:

48 h

Score:

2

Max. score:

4

Reversibility:

fully reversible within: 6d

Irritation parameter:

cornea opacity score

Basis:

animal #2

Time point:

24/48/72 h

Score:

2

Max. score:

4

Reversibility:

fully reversible within: 6d

Irritation parameter:

cornea opacity score

Basis:

animal #3

Time point:

24/48/72 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 6 d

Irritation parameter:

chemosis score

Basis:

animal #1

Time point:

24/48/72 h

Score:

2

Max. score:

4

Reversibility:

fully reversible within: 72h

Irritation parameter:

chemosis score

Basis:

animal #2

Time point:

24/48/72 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: 13 d

Irritation parameter:

chemosis score

Basis:

animal #3

Time point:

24/48/72 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: 13 d

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

24/48/72 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: 13d

Irritation parameter:

conjunctivae score

Basis:

animal #2

Time point:

24/48/72 h

Score:

3

Max. score:

3

Reversibility:

not fully reversible within: 13d

Irritation parameter:

conjunctivae score

Basis:

animal #3

Time point:

24/48/72 h

Score:

3

Max. score:

3

Reversibility:

not fully reversible within: 13d

Interpretation of results:

Category 2 (irritating to eyes) based on GHS criteria

Conclusions:

After the individual introduction of 0.1 g test substance into the conjunctival sac of 3 rabbits, changes occurred in the cornea, iris and conjunctiva. After application of the test substance, the cornea showed slight diffuse turbidity (rating number 1) or details of the iris were slightly shaded due to the clouding (rating number 2). The turbidity zone covered almost the entire corneal area in all animals. On the 6th day of observation, these changes had subsided. The iris showed moderate circular corneal hyperemia during the first three days of observation (rating number 1). The conjunctiva reacted in all animals with diffuse flesh-colored redness (rating number 3). In addition, there was significant swelling, which led to the partial turning of the lids or to half-closed lids (rating number 3). Redness was longest until 13th observation day demonstrable. Hypersecretion, which moistened the eyelids and hair or considerable parts of the eye, was p. appl. to observe. The irritation index is 40. According to this, 3-mercapto-1,2,4-triazole has moderately irritating properties in the rabbit eye. Systemic-toxic effects did not occur after conjunctival application of the test product. The general condition of the experimental animals was undisturbed.

Executive summary:

The substance was tested for its eye-irritating properties on rabbits.0.1 g was placed in the conjunctival sac of 3 experimental animals. Observed changes: Cornea: Slight opacity or turbidity, in which details of the iris were slightly shadowed, affected the entire corneal area. Iris: moderate circulatory corneal hyperemia. Conjunctiva: diffuse flesh-colored redness;clear Swelling, which led to the partial turning of the lids or to the half-closed lids. Other eye changes: Hypersecretion, which moistened the surrounding eyelids and hair or considerable parts around the eye. These findings were no longer than 2 weeks p.appl.demonstrated. The determined stimulus index 40. For 3-mercapto-1, 2, 4-triazole, this results in moderately irritating properties in the rabbit eye. Systemic-toxic effects were not detectable.

Endpoint conclusion

Endpoint conclusion:

adverse effect observed (irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no study available

Justification for classification or non-classification

CLP ((EG) Nr. 1272/2008) criteria for skin irritation/corrosion are not met. A classification is not necessary

CLP ((EG) Nr. 1272/2008) criteria for eye irritation/corrosion are met. Due to the eye irritation score in the key in vivo study the substance is classified in Category 2.