1,2,4-triazole-3-thiol

Endpoint summary

Description of key information

3 -Mercapto-1,2,4 -triazole was administered one-time orally to rats to determine its acute toxicity. The test product was present as a crystalline substance and was applied in an aqueous tragacanth suspension (0.5%).Using application volumes of 4.64 and 10.0 ml / kg with test substance contents of 215 to 464 mg / ml, the dosis in the male and female animals ranged from 1000 to 4640 mg / kg. The intoxication was characterized by tremor, clonic convulsions, loss of muscle tone, generalized reflex failure, diarrhea and poor general condition. Occasionally, stilt walk, ruffled fur, sunken flanks and red-colored tears flow and strenuous breathing were observed. Initial symptoms of poisoning occurred 1 hour after substance administration and lasted up to 5 days. Deaths occurred between 2 hours and 5 days p.appl. A dose of 1000 mg / kg caused no signs of intoxication in female rats.

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records

Reference

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

not specified

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

water and food ad libitum;

Route of administration:

oral: gavage

Vehicle:

water

Remarks:

binder suspension (0.5%)

Details on oral exposure:

food withdrawal 16 h before application

Doses:

1000 / 1470/ 2150/ 4640 mg/kg

No. of animals per sex per dose:

5

Details on study design:

daily obervasion of animals, observation period 14 days

Statistics:

95% probit analysis

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1 353 mg/kg bw

Based on:

test mat.

Sex:

female

Dose descriptor:

LD50

Effect level:

1 457 mg/kg bw

Based on:

test mat.

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

1 297 mg/kg bw

Based on:

test mat.

Mortality:

Dosis (mg/kg) death rate
male 1000 1/5
1470 3/5
2150 5/5
4640 5/5

female 1000 0/5
1470 3/5
2150 5/5
4640 5/5

Clinical signs:

other: The poisoning pattern in the two low dose groups was characterized by diarrhea and poor general health. Occasionally, moderate tremors, ruffled fur, stilts and sunken flanks were observed. After application of a dose of 1000 mg / kg, there were no symptom

Gross pathology:

In the autopsy of the deceased animals and intestinal mucosa and in 2 animals additionally the cecum reddened. The intestine contained aqueous, occasionally red-colored fluid. In one animal, the stomach or intestine was bloated. The spleen of one female animal in the lowest dose group was littered with 1 mm white bumps.

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

LD 50 male: 1279 mg/kg

Executive summary:

3 -Mercapto-1,2,4 -triazole was administered one-time orally to rats to determine its acute toxicity.The test product was present as a crystalline substance and was applied in an aqueous tragacanth suspension (0.5%).Using application volumes of 4.64 and 10.0 ml / kg with test substance contents of 215 to 464 mg / ml, the dosis in the male and female animals ranged from 1000 to 4640 mg / kg.
The intoxication was characterized by tremor, clonic convulsions, loss of muscle tone, generalized reflex failure, diarrhea and poor general condition.Occasionally, stilt walk, ruffled fur, sunken flanks and red-colored tears flow and strenuous breathing were observed.
Initial symptoms of poisoning occurred 1 hour after substance administration and lasted up to 5 days.Deaths occurred between 2 hours and 5 days p.appl.
A dose of 1000 mg / kg caused no signs of intoxication in female rats.

Endpoint conclusion

Endpoint conclusion:

adverse effect observed

Dose descriptor:

LD50

Value:

1 297 mg/kg bw

Acute toxicity: via inhalation route

Endpoint conclusion

Endpoint conclusion:

no study available

Acute toxicity: via dermal route

Endpoint conclusion

Endpoint conclusion:

no study available

Justification for classification or non-classification

CLP ((EG) Nr. 1272/2008) criteria for acute toxicity are met. Based on the LD 50 (male: 1297 mg/kg) the substance is classified in Category 4.