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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2010
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP, guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report date:
2011

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
See chapter 11 of the final report
GLP compliance:
yes (incl. QA statement)
Remarks:
in the final report

Test material

Constituent 1
Chemical structure
Reference substance name:
ethyl 2-cyano-3-oxobutanoate
EC Number:
700-172-3
Cas Number:
634-55-9
Molecular formula:
C7 H9 NO3
IUPAC Name:
ethyl 2-cyano-3-oxobutanoate

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Temperature 20 + 2 °C

Observation 24 and 48 hours

Away from light

Treatments : 1, 10 and 100 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The main study was performed using three concentrations containing 1, 10 and 100 mg/L. Twenty daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.
The concentration 100 mg/L showed 10% immobilisation. As stated in the guideline, this is considered as uncritical.
None of the animals were immobilised in the control.
The 24h-EC50i of potassium dichromate was tested in a current positive control test. The value was determined as 1.6 mg/L, lying within the demanded range of 0.6 – 1.7 mg/L.

The following results were determined for the test item ETHYL-2-CYANO-3-OXOBUTANOATE (species: Daphnia magna).

24h-NOEC = 100 mg/L
48h-NOEC = 100 mg/L
24h-EC50i > 100 mg/L
48h-EC50i > 100 mg/L