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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994-01-05 to 1994-03-30
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Asahi Glass Co., Ltd. (Japan); Batch no. 930817
- Expiration date of the lot/batch: not specified
- Purity test date: 1993-12-20 (before initiation of exposure)

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: cold dark storage place
- Stability under test conditions: stable during exposure period


FORM AS APPLIED IN THE TEST (if different from that of starting material): Colourless and Transparent Liquid
Details on sampling:
- Sampling intervals/frequency for test organisms: The test concentrations in fish were analyzed 4 times (weeks 2, 4, 6, and 8 (n = 2)). Control fish were analyzed at the initiation and termination of exposure (n = 2).
- Sampling intervals/frequency for test medium samples: Concentrations of the test substance in the test water for Level 1 and Level 2 were analyzed 16 times at twice a week intervals (n = 1).
- Sample storage conditions before analysis: Stock solutions were refrigerated and supplied to the test tank.
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods): Please see Section 13.6 of attached study report for further details.
Vehicle:
yes
Remarks:
ion-exchanged water
Details on preparation of test solutions, spiked fish food or sediment:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
A] Dispersant: Test substance and MEGAFAC F142-D (10 times the amount of the test substance) were mixed. The mixture was then diluted with ion-exchanged water to prepare 1000 mg/L stock solution.
B] Level 1: 20 mg/L stock soution was prepared as described in Bullet # A above
Level 2: 2 mg/L stock soution was prepared as described in Bullet # A above
Control: MEGAFAC F142-D was dissolved in ion-exchanged water to prepare 200 mg/L stock solution
- Controls: MEGAFAC F142-D
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): MEGAFAC F142-D
Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: Carp
- Strain: not specified
- Source: Sugishima Fish Farm (Japan)
- Age at study initiation (mean and range, SD): Not specified
- Length at study initiation (length definition, mean, range and SD): average length = 9.7 cm
- Weight at study initiation (mean and range, SD): average weight = 25.5 grams
- Weight at termination (mean and range, SD): Not specified
- Method of breeding: Not specified
- Lipid content at test initiation (mean and range, SD): average = 3.7%
- Health status: Fish were checked visually at delivery and those demonstrating abnormalities were removed. Fish were reared for 2 days in a flow through system following external disinfection.
- Description of housing/holding area: Fish were kept in a 100 L glass tank using a flow through system
- Feeding during test
- Food type: Pelleted feed for carp (Nippon Formula Feed Mfg. Co., Ltd.)
- Amount: Amount corresponding to about 2% of total body weight
- Frequency: Twice a day in halves

ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): After rearing, fish were medicated to eliminate parasites and transferred to an acclimatizing aquarium. Fish demonstrating abnormialties during this period were removed and reared for 36 days in a flow through system at a temperature of 25 ± 2°C. The fish were then transferred to test tanks and reared at the same temperature in the flow through system for another 22 days, following the external disinfection.
- Type and amount of food: Pelleted feed for carp (Nippon Formula Feed Mfg. Co., Ltd.); Amount corresponding to about 2% of total body weight. On the day before sampling no feed was provided.
- Feeding frequency: Twice a day in halves
- Health during acclimation (any mortality observed): Not specified
Route of exposure:
feed
Justification for method:
other: Not specified
Test type:
flow-through
Water / sediment media type:
natural water
Remarks:
Underground water
Total exposure / uptake duration:
ca. 8 wk
Hardness:
112 mg/L
Test temperature:
25 ± 2°C
pH:
7.4 at 20°C
Dissolved oxygen:
Level 1: 4.7 - 6.3 mg/L
Level 2: 4.7 - 6.4 mg/L
Control: 6.2 - 6.9 mg/L
TOC:
Not determined
Conductivity:
388 µs/cm
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 L glass tank for volatile substances
- Type (delete if not applicable): open / closed (not specified)
- Material, size, headspace, fill volume: 100 L Glass tank
- Aeration: not specified
- Type of flow-through (e.g. peristaltic or proportional diluter): not specified
- Renewal rate of test solution (frequency/flow rate): 2 mL/min for stock solution and 800 mL/min for dilution water; 1155 liters/day for test water were supplied
- No. of organisms per vessel: Level 1 and 2: 12; Control: 5
- No. of vessels per concentration (replicates): Not specified
- No. of vessels per control / vehicle control (replicates): Not specified
- Biomass loading rate: Not specified

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Particulate matter: <1 mg/L
- Chlorine: not determined
- Alkalinity: 94.0 mg/L
- Conductance: 388 µs/cm
- Holding medium different from test medium: No
- Intervals of water quality measurement: Concentration of the test substance in the test water for Level 1 and Level 2 were analyzed 16 times (twice a week interval; n = 1)
- Intervals of test medium replacement: Not specified

OTHER TEST CONDITIONS
- Adjustment of pH: Not specified
- Photoperiod: Not specified
- Light intensity: Not specified
- For OECD 305 part III (dietary exposure fish bioaccumulation), overall daily feeding rate used in the study: amount corresponding to about 2% of total body weight
- For OECD 305 part III (dietary exposure fish bioaccumulation), number of feeds per day (number of feeds daily ration split between): Twice a day in halves

RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations: 125 mg/L
- Results used to determine the conditions for the definitive study: Yes; 48-hour LC50 >125 mg/L
- Other justification for choice of test concentrations: Based on preliminary test results for 48-hour LC50 and analytical detection limits
Nominal and measured concentrations:
Nominal Concentrations
High exposure = 50 µg/L
Low exposure = 5 µg/L
Details on estimation of bioconcentration:
Bioconcentration Factors were calculated using equations provided in Tables 7, 8, and 9 of the attached study report and rounded off to 3 figures.
Key result
Conc. / dose:
ca. 50 µg/L
Temp.:
>= 23 - <= 27 °C
pH:
7.4
Type:
BCF
Value:
>= 293 - <= 615 dimensionless
Basis:
not specified
Key result
Conc. / dose:
ca. 5 µg/L
Temp.:
>= 23 - <= 27 °C
pH:
7.4
Type:
BCF
Value:
>= 336 - <= 658 dimensionless
Basis:
not specified

Measured concentrations of test substance in test water (avg value at times from start of exposure)

 

2 Weeks

4 Weeks

6 Weeks

8 Weeks

Level 1

50.5

49.1

48.4

49.2

Level 2

4.56

4.49

4.37

4.38

 

Bioconcentration Factors

 

2 Weeks

4 Weeks

6 Weeks

8 Weeks

Level 1

239

387

487

527

308

615

478

540

Level 2

482

658

474

534

336

524

354

496

Validity criteria fulfilled:
not specified
Conclusions:
The BCF's of the test substance ranged from 293 to 615 in Level 1 and from 336 to 658 in Level 2.
Executive summary:

The BCF's of the test substance ranged from 293 to 615 in Level 1 and from 336 to 658 in Level 2.

No abnormality in behaviour or appearance of the test fish was observed. Each average concentration of the test substance in test water was maintained at more than 90% of each of the nominated concentrations.

Description of key information

Bioconcentration factors (BCFs) in fish ranged from 293 to 615 (50 µg/L) and 336 to 658 (5 µg/L).

Key value for chemical safety assessment

Additional information

In a GLP-compliant bioaccumulation study in fish (equivalent to OECD Guideline 305 C), Carp (Cyprinus carpio) were exposed to 1,1,1,2,2,3,3,4,4,5,5,6,6-tridecafluorohexane for 8 weeks at concentrations of 5 µg/L or 50 µg/L (Kurume Research Laboratories, 1994b). No abnormality in behaviour or appearance was observed. Bioconcentration factors (BCFs) ranged from 293 to 615 (50 µg/L) and 336 to 658 (5 µg/L).