3-(Hydroxymethyl)-1-pentene

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14.12.2007-11.03.2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Storage of Samples:
The samples were delivered frozen to the IBACON Laboratory. The sample containers were not damaged. All samples were stored in a freezer (- 10 °C), protected from light until analysis was performed.

Fortified Samples:
About 50 mg of the test item were dissolved in 50 mL acetonitrile to obtain a stock solution of I g test item/L, respectively. Appropriate amounts of this stock solution were diluted with test medium to obtain fortified samples at a level of 10 and 100 mg test item/L. Exact values were documented in the raw data.

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: The daphnids introduced in the test were taken from in-house laboratory culture.
- Age of parental stock: <=24 h

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

14°dH (ca. 250 mg/L CaCO3)

Test temperature:

20 - 21°C

pH:

7.84 - 7.91

Dissolved oxygen:

93.9 - 96.8%

Nominal and measured concentrations:

Nominal Concentration: 100 mg/L
Measured: 100% at geginning and and 96% of nominal at end of test

Details on test conditions:

TEST SYSTEM
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to ELENDT (1990)

OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h dark

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

After 48 hours of exposure no immobilisation of the test animals was observed.

Influence of the test material on the Mobility of Daphnia magna

	Mean analytical concentration during the study		Immobilized/exposed Daphnia magna
Nominal Concentration [mg/L]	[%]	[mg/L]	24 hours	48 hours
0	-	-	0/20	0/20
100	98	97.6	0/20	0/20

A nominal concentration of 100 mg/L of the test item revealed no aquatic toxicity in this test system. No remarkable observations were made concerning the appearance of the solution of the test material preparation. The test medium was a clear preparation and stayed unchanged throughout the study.

Validity criteria fulfilled:

yes

Conclusions:

The 96h-LC50 was determined to be >100 mg/L (nominal).

Executive summary:

Study design

The objective of this study was to determine the acute toxicity of the test material using Daphnia magna. For this purpose, juvenile Daphnia magna were exposed to an aqueous test material solution over 48 hours, under defined conditions. The study comprised of four test vessels per concentration containing five Daphnia magna each, i.e. 20 Daphnia magna per concentration (test medium group) and control group. Daphnia magna were exposed to a nominal test material concentration of 100 mg/L (limit test) in a closed static system.

Results

The analytical recovery was 100 and 96 % after 0 and 48 hours, respectively. The mean recovery over 48 hours was 98 %. As the stability of the test material in the aqueous solution was ≥ 80 %, the EC₅₀ values were calculated with the nominal concentrations. Nominal, corresponding mean analytical concentrations, and number of immobilized daphnia are given in the following table.

	Mean analytical concentration during the study		Immobilized/exposed Daphnia magna
Nominal Concentration [mg/L]	[%]	[mg/L]	24 hours	48 hours
0	-	-	0/20	0/20
100	98	97.6	0/20	0/20

Daphnia magna exposed to an aqueous preparation of the nominal concentration of 100 mg/L of the test item were not affected.

Conclusion

No immobilized daphnids were observed during the test period. The 96h-LC50 was determined to be >100 mg/L.

Description of key information

The 96h-LC50 was determined to be >100 mg/L.