3-(Hydroxymethyl)-1-pentene

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12.12.2006-19.12.2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

1992

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Version / remarks:

1992

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-ethylbut-3-en-1-ol

EC Number:

610-949-8

Cas Number:

53045-70-8

Molecular formula:

C6H12O

IUPAC Name:

2-ethylbut-3-en-1-ol

Test material form:

liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: sewage plant Rossdorf, Germany
The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and again centrifuged. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 1.5 g dry material per litre were mixed with test water and then aerated until use overnight.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

ca. 103 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: reconstituted water:
- Test temperature: 22°C
- pH: 7.6 - 7.9
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: Manometric Test System, 500 mL
- Number of culture flasks/concentration: 2

CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 flasks
- Abiotic sterile control: 1 flask
- Toxicity control: 1 flask
- Other: procedure control 1 flask

The amounts of test item and reference item were directly weighed into the test flasks. No emulsifiers or solvents were used, but the solutions were dispersed by stirring to achieve a homogeneous solution of the test item. The closed test flasks were incubated in a climatic chamber
under continuous stirring.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

47

Sampling time:

14 d

% Degradation 2

Key result

Parameter:

% degradation (O2 consumption)

Value:

72

Sampling time:

28 d

Details on results:

10-day window began on day 5 and reached 49% degradation at the end.
The reference substance degraded to 87% after 14 days and to 90° after 28 days.
In the toxicity control, 42% degradation were noted within 14 days and 56% degradation within 28 days.

BOD5 / COD results

Results with reference substance:

The reference item sodium benzoate was sufficiently degraded to 87 % after 14 days and to 90 % after 28 days of incubation.The reference item sodium benzoate was sufficiently degraded
to 87 % after 14 days and to 90 % after 28 days of incubation.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable, but failing 10-day window

Conclusions:

In a manometric respirometry test according to OECD 301F the test item degraded to 47% after 14 days and to 72% after 28 days. At the end of the 10-day window 49% degradation were observed. Therefore, the substance is classified as readily degradable, but failing the 10-day window.

Executive summary:

Purpose

The test item was investigated for its ready biodegradability in a Manometric Respirometry Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. The GLP study was performed according to OECD TG 301F.

 

Result

Biodegradation of test item

Under the test conditions the test item degraded to 47% after 14 days and to 72% after 28 days. At the end of the 10-day window 49% degradation were observed. Therefore, the substance is considered as readily degradable, but failing the 10-day window.

 

Biodegradation of Sodium Benzoate

The reference item sodium benzoate was sufficiently degraded to 87 % after 14 days and to 90 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.

 

Biodegradation in the Toxicity Control

In the toxicity control containing both, the test item and the reference item 42 % biodegradation was noted within 14 days and 56 % biodegradation was determined after 28 days of incubation. According to the test guidelines the test item can be assumed to be not inhibitory on the activated sludge micro organisms because degradation was >25 % within 14 days.

 

Conclusion

The test is considered as readily degradable, but failing the 10-day window.