Arctium lappa, ext.

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2008

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study, described in sufficient detail

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Arctium lappa, ext.

EC Number:

281-658-8

EC Name:

Arctium lappa, ext.

Cas Number:

84012-13-5

IUPAC Name:

Arctium lappa, ext.

Details on test material:

- Name of test material (as cited in study report): Klettenfruchtextrakt conc.
- Physical state / colour / odour: viscous liquid, golden brown, fruity odour

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Length at study initiation: 3.1 +- 0.14 cm

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

10.3 °dH, 181 mg CaCO3/L

Test temperature:

20.4 - 21.0°C

pH:

6.8 - 7.8

Dissolved oxygen:

6.2 - 9.0 mg/L

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 7

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water, dechlorinated

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

26 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

134 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CI: 95 - 210 mg/L

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

338 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Abnormal responses: none

Reported statistics and error estimates:

Probit analysis

Any other information on results incl. tables

Sublethal observations / clinical signs:

Results:

Concentration [mg/L]	641.3	337.5	177.7	93.5	49.2	25.9	Control
Mortality after 24 h [%]	100	57	0	0	0	0	0
Mortality after 48 h [%]	100	57	0	0	0	0	0
Mortality after 72 h [%]	100	57	0	14	0	0	0
Mortality after 96 h [%]	100	100	57	57	14	0	0

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

In a 96-h acute toxicity study, Danio rerio were exposed to Arctium lappa extract at concentrations of 0, 25.9, 49.2, 93.5, 177.7, 337.5, and 641.3 mg/L under static conditions in dechlorinated drinking water as medium. The 96-h LC₅₀ based on mortality was 134 mg/L. Abnormal responses were not observed.

This toxicity study is classified as acceptable and satisfies the guideline requirement for toxicity study on fish.

 

Results Synopsis

Test organism size: 3.1 +- 0.14 cm

Test Type: Static

LC₅₀: 134 mg L                         95% C.I.: 95 - 210 mg L

Endpoint Effected: Mortality