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Short term toxicity to aquatic invertebrate

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to invertebrate was predicted trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6). Intoxication value was estimated to be 1399.30 mg/l for Daphnia magna for 48  hrs duration. It was concluded that trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6)  was likely to be not toxic to aquatic invertebreate.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary

descriptor and considering the closest read across substances, the toxicity to algae was predicted trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6). Growth rate inhibition value was estimated to be 115.066 mg/l for Pseudokirchneriella subcapitata for 72  hrs duration. It was concluded that trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6) was likely to be not toxic to aquatic algae.

Additional information

Short term toxicity to aquatic invertebrate

Following studies include the experimental studies for the structurally similar read across to conclude the toxicity extent of trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6) towards aquatic invertebrate is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to invertebrate was predicted trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6). Intoxication value was estimated to be 1399.30 mg/l for Daphnia magna for 48  hrs duration. It was concluded that trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6)  was likely to be not toxic to aquatic invertebreate.

The predicted data of target chemical is supported by the structurally similar read across Succinic acid (CAS: 110 -15 -6) from the publication Journal of the Water Pollution Control Federation 1980, suggests that The acute static tests were conducted as described in Ecological Research Series, EPA-600/3-75-009 ("Methods for Acute Toxicity Tests with Fish, Macroinvertebrates, and Amphibians"). First instar Daphnia were used for all tests. The tests were conducted in duplicate for 48 hours at 22°C in a constant-temperature chamber. All test substance concentrations were nominal. 

The Effective concentration to 50% of Daphnia magna is 374.2 mg/l at which 50% immobility was observed.

On the basis of the value obtained it can be concluded that the Succinic acid is not toxic to Daphnia magna at environmentally relevant concentrations and can be considered as “not Classified” under CLP regulation.

Further it is supported by another structurally similar read across Malonic acid(CAS: 141-82-2) from the similar publication Journal of the Water Pollution Control Federation 1980, also suggests thatIn Short term toxicity test, Daphnia magna was exposed to Malonic acid for 48 hrs.The effective concentration EC50 value for Daphnia magna when exposed to Malonic acid for 48 Hour is 275 mg/l.At which 50 % immobility was observed.On the basis of obtained value it can be concluded that the Malonic acid is not toxic to Daphnia magna and is considered to be “not classified” under CLP regulation.

Thus based on the effect concentrations which is in range of 275 mg/l to 1399.30 mg/L gives the conclusion that test substance trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6) was likely to be not toxic to invertebrate at environmentally relevant concentrations and applying weight of evidence approach it can be considered to benot classifiedas per the CLP classification criteria for aquatic environment.

Toxicity to aquatic algae and cyanobacteria

Following studies include the experimental studies for the structurally similar read across to conclude the toxicity extent of trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6) towards aquatic algae is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary

descriptor and considering the closest read across substances, the toxicity to algae was predicted trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6). Growth rate inhibition value was estimated to be 115.066 mg/l for Pseudokirchneriella subcapitata for 72  hrs duration. It was concluded that trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6) was likely to be not toxic to aquatic algae.

Similar prediction is done for the target chemical by using the three different models i.e, Battery, Leadscope and SciQSAR used within Danish QSAR database, the short term toxicity on green algae was predicted for test substancetrisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl}(CAS:6598 -63 -6). The average EC50 value was given by the third model i.e, Battery model. On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substancetrisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} is estimated to be 2809.387 mg/l for Pseudokirchneriella subcapitata during 72 hr exposure duration. Thus, on the basis of EC50 value,it can be concluded that the test chemicaltrisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl}can be considered asnon-toxicto green algae at environmentally relevant concentrations and can be considered "not-classified" as per the CLP classification criteria.

The Predicted data for the target chemical is assissted by the experimental study of structurally similar read acrossMetformin (CAS: 657 -24 -9) from the publication Toxicology Letters 2003,Toxicity to aquatic algae and cyanobacteria assessment was carried out to study the effects of Metformin on Desmodesmus subspicatus. Effective concentration to 50% of Desmodesmus subspicatus at which decrease in population growth rate was measured is 320 mg/L. It can be concluded that the Metformin is not toxic to the aquatic environment and can be considered as “not classified” as CLP regulation.

Similar experimental study of another structurally simialr read acrossBenzoic Acid (CAS: 65 -85 -0) from the publication Journal of Hazardous Materials 2009, suggests that theShort term toxicity of Benzoic acid toPseudokirchneriella subcapitata(green algae) study was carried out for 48 hrs.The study was based on the effects of the test compound onPseudokirchneriella subcapitatain a static fresh water system. On the basis of population of the test organismPseudokirchneriella subcapitata(green algae), the 48 hr EC50 value was determined to be 207.5 mg/l. Thus, based on this value, it can be concluded that the substance Benzoic acid can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

Thus based on the effect concentrations which is in range of 115.066 mg/l to 2809.387 mg/L gives the conclusion that test substance trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6) was likely to be not toxic to algae at environmentally relevant concentrations and applying weight of evidence approach it can be considered to benot classifiedas per the CLP classification criteria for aquatic environment.