Dodecanenitrile

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from authoritative databases.

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Version / remarks:

No data available

Deviations:

not specified

Principles of method if other than guideline:

Determination of short term toxicity and Growth Inhibition of Alga (Pseudokirchneriella subcapitata) due to the exposure of Dodecanenitrile.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: Dodecanenitrile
- Molecular formula: C12H23N
- Molecular weight: 181.321 g/mole
- Smiles notation: C(CCCCCC)CCCCC#N
- InChl: 1S/C12H23N/c1-2-3-4-5-6-7-8-9-10-11-12-13/h2-11H2,1H3
- Substance type: organic
- Physical state: colourless liquid

Analytical monitoring:

not specified

Details on sampling:

No data available

Vehicle:

not specified

Details on test solutions:

- Method: Required amount of test substance was added into the Erlenmeyer flask filled with medium under the water surface to give 100 mg/L (nominal). After stirring for about 48 hours, some amount of the solution was extracted for stock solution to prepare test solutions by mixing and stirring with medium.

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

No data available

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Remarks on exposure duration:

No data available

Post exposure observation period:

No data available

Hardness:

No data available

Test temperature:

22.2 - 22.4◦C

pH:

No data available

Dissolved oxygen:

No data available

Salinity:

No data available

Conductivity:

No data available

Nominal and measured concentrations:

50, 16, 5.0, 1.6, 0.50 % (geometric ratio; square root of 10) [0.48, 0.15, 0.054, 0.016, 0.0051 mg/L as measured concentration], control

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Type (delete if not applicable): No data available
- Material, size, headspace, fill volume: 600 mL/control (100 mL/vessel), 300 mL/concentration (100 mL/vessel), (1 vessel was separately set for analysis at 24 and 48 hours respectively.)
- Aeration: No data
- Type of flow-through (e.g. peristaltic or proportional diluter): No data
- Renewal rate of test solution (frequency/flow rate): Frequency of analysis: at the start of exposure, after 24 hours and 48 hours from start of exposure, at the end of exposure.
- Initial cells density: No data available
- Control end cells density: No data available
- No. of organisms per vessel: No data available
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): No data available

GROWTH MEDIUM : No data available
- Standard medium used: yes/no
- Detailed composition if non-standard medium was used:

TEST MEDIUM / WATER PARAMETERS: No data available
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Sterile test conditions: yes/no
- Adjustment of pH:
- Photoperiod:
- Light intensity and quality: 95 - 99 microE/m2/s
- Salinity (for marine algae):

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : No data
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter]
- Chlorophyll measurement:
- Other:

TEST CONCENTRATIONS: No data available
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

not specified

Key result

Duration:

3 d

Dose descriptor:

EC50

Effect conc.:

0.15 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95 % CL: 0.14 - 0.16 mg/L

Key result

Duration:

3 d

Dose descriptor:

NOEC

Effect conc.:

0.054 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: No effect was observed

Details on results:

No data available

Results with reference substance (positive control):

No data available

Reported statistics and error estimates:

No data available

Validity criteria fulfilled:

not specified

Conclusions:

Based on the reduction and inhibition of growth rate of green algae (Pseudokirchneriella subcapitata), due to the exposure with dodecanenitrile, the EC50 was 0.15 mg/l and the NOEC was 0.054 mg/l.

Executive summary:

Determination of short term toxicity and Growth Inhibition of Alga (Pseudokirchneriella subcapitata) due to the exposure of dodecanenitrile. Study was performed according to the OECD guideline in the static system.

 

Content rate of test stock solution was 50, 16, 5.0, 1.6, 0.50 % (geometric ratio; square root of 10) [0.48, 0.15, 0.054, 0.016, 0.0051 mg/L as measured concentration], with one control. Test solution was prepared in flasks. Required amount of test substance was added into the Erlenmeyer flask filled with medium under the water surface to give 100 mg/L (nominal). After stirring for about 48 hours, some amount of the solution was extracted for stock solution to prepare test solutions by mixing and stirring with medium.

 

Based on the reduction and inhibition of growth rate of green algae (Pseudokirchneriella subcapitata), due to the exposure with Dodecanenitrile, the EC50 was 0.15 mg/l and the NOEC was 0.054 mg/l.

Based on the EC50 value it was concluded that the chemical was toxic and can be consider to be classified in aquatic acute 1 as per the CLP classification criteria.

Description of key information

Determination of short term toxicity and Growth Inhibition of Alga (Pseudokirchneriella subcapitata) due to the exposure of dodecanenitrile. Study was performed according to the OECD guideline in the static system.

 

Content rate of test stock solution was 50, 16, 5.0, 1.6, 0.50 % (geometric ratio; square root of 10) [0.48, 0.15, 0.054, 0.016, 0.0051 mg/L as measured concentration], with one control. Test solution was prepared in flasks. Required amount of test substance was added into the Erlenmeyer flask filled with medium under the water surface to give 100 mg/L (nominal). After stirring for about 48 hours, some amount of the solution was extracted for stock solution to prepare test solutions by mixing and stirring with medium.

 

Based on the reduction and inhibition of growth rate of green algae (Pseudokirchneriella subcapitata), due to the exposure with Dodecanenitrile, the EC50 was 0.15 mg/l and the NOEC was 0.054 mg/l.

Key value for chemical safety assessment

EC50 for freshwater algae:

0.15 mg/L

EC10 or NOEC for freshwater algae:

0.054 mg/L

Additional information

Based on the various experimental data and prediction data for the target chemical study have been reviewed to determine the toxic nature of Dodecanenitrile (2437-25-4) on the growth of algae. The studies are as mentioned below:

In the first experimental key study for Dodecanenitrile (2437-25-4) from j-check, 2017 Determination of short term toxicity and Growth Inhibition of Alga (Pseudokirchneriella subcapitata) due to the exposure of dodecanenitrile. Study was performed according to the OECD guideline in the static system. Content rate of test stock solution was 50, 16, 5.0, 1.6, 0.50 % (geometric ratio; square root of 10) [0.48, 0.15, 0.054, 0.016, 0.0051 mg/L as measured concentration], with one control. Test solution was prepared in flasks. Required amount of test substance was added into the Erlenmeyer flask filled with medium under the water surface to give 100 mg/L (nominal). After stirring for about 48 hours, some amount of the solution was extracted for stock solution to prepare test solutions by mixing and stirring with medium. Based on the reduction and inhibition of growth rate of green algae (Pseudokirchneriella subcapitata), due to the exposure with Dodecanenitrile, the EC50 was 0.15 mg/l and the NOEC was 0.054 mg/l. Based on the EC50 value it was concluded that the chemical was toxic and can be consider to be classified in aquatic acute 1 as per the CLP classification criteria.

 

Similarly in the second supporting predicted study for the target chemical Dodecanenitrile (2437-25-4) taken from the episuite 2017, the short-term toxicity of the test substance Dodecanenitrile (2437 -25 -4) to green algae is predicted using EPI Suite ECOSAR version 1.11. On the basis of effects observed in a static freshwater system during a 96 hr exposure, the effect concentration (EC50) for the substance is estimated to be 0.767 mg/L for green algae. Based on this value, it can be concluded that the test chemical Dodecanenitrile can be considered as toxic to green algae at environmentally relevant concentrations and can be considered to be classified in aquatic acute/chronic 1 category as per the CLP classification criteria.

 

 Similarly in the third supporting study for Dodecanenitrile (2437-25-4) 2003, toxicity was estimated from robust summary SAR model. Determination of short term toxicity and Growth Inhibition of green algae due to the exposure of Dodecanenitrile. Test was performed in static system as per the study for 96hrs. Based on the reduction and inhibition of growth rate of green algae due to the exposure with Dodecanenitrile, the EC50 was 0.24 mg/l. Based on the EC50 value it was concluded that the chemical was toxic and can be consider to be classified in aquatic acute/chronic 1 as per the CLP classification criteria.

Similarly in the fourth supporting study for RA chemical 1-Octanethiol (111-88-6), j-check, Determination of short term toxicity of 1-Octanethiol on the growth of green algae was studied by providing the exposure of chemical for 72hrs. Test was performed by following the standard OECD Guideline 201 (Alga, Growth Inhibition Test). After the 72hrs of exposure interval period, toxicity and growth inhibition was observed. Based on the inhibition of growth rate and area under growth curve (AUG) of green algae due to the exposure of 1-Octanethiol, the EC50 was 0.014 mg/l and 0.086 mg/l. And the NOEC on the basis of growth rate and AUG was 0.0031 mg/l and 0.027 mg/l. Thus based on the EC50, chemical 1-Octanethiol was consider as toxic and can be consider to be classified as aquatic acute/chronic 1 category as per the CLP classification criteria.

Based on the data obtain from various experimental studies for the immobility due to the exposure of Dodecanenitrile thus it was concluded that the chemical  Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute/chronic 1 category as per CLP classification criteria.