Dodecanenitrile

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from authoritative databases.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

No data available

Deviations:

not specified

Principles of method if other than guideline:

Determination of acute short term toxicity Test of Dodecanenitrile on the growth of fish Oryzias latipes.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: Dodecanenitrile
- Molecular formula: C12H23N
- Molecular weight: 181.321 g/mole
- Smiles notation: C(CCCCCC)CCCCC#N
- InChl: 1S/C12H23N/c1-2-3-4-5-6-7-8-9-10-11-12-13/h2-11H2,1H3
- Substance type: organic
- Physical state: colourless liquid

Analytical monitoring:

yes

Details on sampling:

No data available

Vehicle:

not specified

Details on test solutions:

- Method: The test substance was added to the preparation vessel filled with test water to give 100 mg/L (nominal). The vessel was sealed and stirred gently for 48 hours, and then left standing for about 1 hour. After that, some amount of the solution in middle layer was extracted for stock solution to prepare test solutions by dilution of it.

Test organisms (species):

Oryzias latipes

Details on test organisms:

- Feeding during test: No

Test type:

semi-static

Water media type:

other: Dechlorinated tap water

Total exposure duration:

96 h

Remarks on exposure duration:

Extended to 120 hours because of poor solubility of the test substance.

Post exposure observation period:

No data available

Hardness:

No data available

Test temperature:

23.2 - 24.0 ◦C

pH:

No data available

Dissolved oxygen:

No data available

Salinity:

No data available

Conductivity:

No data available

Nominal and measured concentrations:

100, 56, 31, 17, 9.5 % (geometric ratio 1.8) [1.3, 0.65, 0.35, 0.19, 0.096 mg/L as measured concentration], control

Details on test conditions:

TEST SYSTEM
- Test vessel: No data
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Test vessel filled with 5 liter of solution per concentration.
- Aeration: No
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): Every 24 hours
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): No data available
- Biomass loading rate: No data available

TEST MEDIUM / WATER PARAMETERS: No data available
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: Room light, 16 hours light / 8 hours dark
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : No data available

TEST CONCENTRATIONS: No data available
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.84 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Toxic to fishes

Details on results:

- Behavioural abnormalities: Mortality was observed at the concentration of 0.84 mg/l.

Results with reference substance (positive control):

No data available

Reported statistics and error estimates:

No data available

Sublethal observations / clinical signs:

- Lowest concentration causing 100 %   

 mortality (96 h): 1.3 mg/L

- Highest concentration causing 0 % mortality (96 h): 0.35 mg/L,

(All results were values based on geometric mean values of the measured concentrations.)

Validity criteria fulfilled:

not specified

Conclusions:

Based on the mortality of fish Oryzias latipes due to the exposure of Dodecanenitrile, the LC50 was 0.84 mg/l.

Executive summary:

Determination of acute short term toxicity Test of Dodecanenitrile on the growth of fish Oryzias latipes. Test was performed according to the standard OECD guideline 203.

 

Various concentrations was prepared for the test is 0, 1.3, 0.65, 0.35, 0.19, 0.096 mg/L.The test substance was added to the preparation vessel filled with test water to give 100mg/L (nominal). The vessel was sealed and stirred gently for 48 hours, and then left standing for about 1 hour. After that, some amount of the solution in middle layer was extracted for stock solution to prepare test solutions by dilution of it. After the 96hrs of exposure with chemical mortality was observed. 

 

Based on the mortality of fish Oryzias latipes due to the exposure of Dodecanenitrile, the LC50 was 0.84 mg/l. Thus chemical Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.

Description of key information

Determination of acute short term toxicity Test of Dodecanenitrile on the growth of fish Oryzias latipes. Test was performed according to the standard OECD guideline 203.

 

Various concentrations was prepared for the test is 0, 1.3, 0.65, 0.35, 0.19, 0.096 mg/L.The test substance was added to the preparation vessel filled with test water to give 100mg/L (nominal). The vessel was sealed and stirred gently for 48 hours, and then left standing for about 1 hour. After that, some amount of the solution in middle layer was extracted for stock solution to prepare test solutions by dilution of it. After the 96hrs of exposure with chemical mortality was observed. 

 

Based on the mortality of fish Oryzias latipes due to the exposure of Dodecanenitrile, the LC50 was 0.84 mg/l. Thus chemical Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.84 mg/L

Additional information

Based on the various experimental data and prediction data for the target chemical study have been reviewed to determine the toxic nature of Dodecanenitrile (2437-25-4) on the growth of fishes. The studies are as mentioned below:

In the first experimental key study for Dodecanenitrile (2437-25-4) from j-check, 2017 Determination of acute short term toxicity Test of Dodecanenitrile on the growth of fish Oryzias latipes. Test was performed according to the standard OECD guideline 203. Various concentrations was prepared for the test is 0, 1.3, 0.65, 0.35, 0.19, 0.096 mg/L. The test substance was added to the preparation vessel filled with test water to give 100mg/L (nominal). The vessel was sealed and stirred gently for 48 hours, and then left standing for about 1 hour. After that, some amount of the solution in middle layer was extracted for stock solution to prepare test solutions by dilution of it. After the 96hrs of exposure with chemical mortality was observed. Based on the mortality of fish Oryzias latipes due to the exposure of Dodecanenitrile, the LC50 was 0.84 mg/l. Thus chemical Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.

 

In the another experimental supporting study for Dodecanenitrile (2437-25-4), Toxicology Methods, 1997, The “gold standard” of fish toxicity testing is the 96 h flow-through fathead minnow (Pimephales promelas) 50% mortality database established by the U.S. Environmental Protection Agency. In the current study toxic potencies of chemicals measured in both tetratox and the 96-h Pimephales promelas lethality assay are compared. The Lethal concentration causing 50% mortality (LC50) of fathead minnow when exposed to Dodecanenitrile for 96 hours under the test conditions is 2.344 mg/l. It is reported to be bioactive.

Similarly in the third supporting study for the Dodecanenitrile (2437-25-4), ACD lab 2017, According to aquatic toxicity module i.e ACD/LabsI-Lab 2.0;Algorithm Version: v5.0.0.184 the lethal concentration (LC50) of the test substance Dodecanenitrile was predicted to be 0.28 mg/l on fish species Fathead minnow (Pimephales promelas). Based on the LC50 calculation the estimated Reliability Index (RI) of test chemical Dodecanenitrile predicted to be 0.28 mg/. As per model substance have moderate and high reliability ifRI > 0.5).Thus reported RII value indicate it has high reliability. And LC50 value indicate that the test substance Dodecanenitrile considered as toxic, harmful to aquatic organisms and thus can be classified as aquatic acute 1 and hazardous as per the CLP criteria.

In the fourth supporting predicted study for the Dodecanenitrile (2437-25-4), from EPIsuite, Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic fish was predicted. On the basis of this program, the LC 50 value for short term toxicity to aquatic fish was predicted to be 0.489 mg/l for Dodecanenitrile in 96 hrs. Based on this value it can be concluded that the substance Dodecanenitrile is considered to be toxic to aquatic environment and can be classified in aquatic acute 1/chronic 1 category as per the criteria mentioned in CLP regulation.

Similarly in the fifth supporting study for the Dodecanenitrile (2437-25-4), ecotox 2017, toxicity was measured. Determination of acute short term toxicity Test of Dodecanenitrile on the growth of Pimephales promelas (Fathead Minnow) was carried out for 96hrs. Test was performed in the static system by maintaining the proper temperature and Ph. After the 96hrs of exposure with chemical mortality and toxic symptoms was observed. Based on the mortality of fish Pimephales promelas (Fathead Minnow) due to the exposure of Dodecanenitrile, the LC50 was 0.43 mg/l. Thus chemical Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.

 

Similarly in the sixth supporting study for Dodecanenitrile (2437-25-4) 2003, toxicity was measured. Determination of short term toxicity and mortality of Zebra fish (Brachydanio rerio) due to the exposure of Dodecanenitrile. Test was performed as per the standard OECD guidelines. 96hrs of exposure were given to the Zebra fishes by performing in static system. No fish died at 1 mg/l and 100 % of the fish died at 10 mg/l. Based on the mortality of Zebra fish (Brachydanio rerio) due to the exposure with Dodecanenitrile, the EC50 was > 1 mg/l. Based on the EC50 value it was concluded that the chemical was toxic and can be consider to be classified in aquatic chronic 2 as per the CLP classification criteria.

 

Based on the data obtain from various experimental studies and predicted study for the mortality of fishes due to the exposure of Dodecanenitrile thus it was concluded that the chemical Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.