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EC number: 277-616-3
CAS number: 73816-74-7
The test substance, Reactive Red 45:1,
was tested forthe ready biological degradability in Closed Bottle Test.
The test was performed according to:
Method C.4E - Closed Bottle Test,
Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
The results of biological degradation
are related to experimentally determined COD values
of the test and reference substance at the beginning of the test.
The test substance was sufficiently
soluble in used mineral medium so the dosage from the stock solution was
Sodium benzoate was used as the
reference substance. The dosage was carried out from the stock solution.
COD of the test substance in medium at
the beginning of the main test: 3.40 mg·L-1
COD of the reference substance in
medium at the beginning of the main test: 3.32 mg·L-1
In parallel to the main test the
toxicity test was performed.
Based on the test substance contains
nitrogen in the chemical composition, the oxidized nitrogen forms were
determined and the correction for nitrification was carried out.
The test was performed at temperature
of 20 ± 1 °C with the pH values of solutions 7 - 8
at the beginning of the test.
Validity of the test
The prescribed validity criteria in
the test were fulfilled.
substance was not inhibiting for the used inoculum.
all criteria of acceptability were met, this study is considered to be
study of ready biological degradabilitythe
degradation of 7.1 % of the test substance, Reactive
Red 45:1, was
attained in the end of study.
Reactive Red 45:1 is considered to be not
readily biodegradable under the test condition.
In the 28-day study of ready
biodegradability (Closed bottle test), the degradation of the test
substance, Reactive Red 45:1, was 7.1% at the end of the study.
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