6-aminonaphthalene-1,3-disulphonic acid

Endpoint summary

Description of key information

Short term toxicity to fish:

Toxic effects of test substance 6 amino-1,3 naphthalene –disulfonic acid was determined on various fish species like Leuciscus idus, Brachydanio rerio, Salmo gaidneri, Pimephales promelas, Lepomis macrochirus, Poecilia reticulate for 96 hrs in static condition and in fresh water. During experiment the lethal concentration (LC0) value was1000 mg/l. Thus based on the above experimental result the test substance6 amino-1,3 naphthalene –disulfonic acid was nontoxic to fishes and thus not consider for the further classification as per the CLP criteria.

Short term toxicity to aquatic invertebrates:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on Daphnia magna was predicted for 118-33-2 (1, 3-Naphthalenedisulfonic acid, 6-amino). The EC50 value was estimated to be 138mg/l when1, 3-Naphthalenedisulfonic acid, 6-amino exposed to Daphnia magna for 48hrs.

Toxicity to aquatic algae and cyanobacteria:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on Desmodesmus subspicatus was predicted for 118-33-2 (1, 3-Naphthalenedisulfonic acid, 6-amino). The EC50 value was estimated to be 121.060mg/l when1, 3-Naphthalenedisulfonic acid, 6-amino exposed to Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) for 72hrs.

Toxicity to microorganisms:

Based on the key study from chemosphere, Toxic effects of test substance6 amino-1 ,3 naphthalene –disulfonic acid was determined on bacteriafor 24 hrs in static condition and in fresh water.During experiment the effect concentration (EC0) value was1000 mg/l. Based on the experimental data the effect concentration (EC0) of test substancesubstance 6 amino-1, 3 naphthalene –disulfonic acid for bacteria was 1000mg/l. And thus the chemical was non toxic and not classified as per the CLP classification criteria.

Additional information

Short term toxicity to fish:

Based on the various experimental data for the target chemical 6 amino-1,3 naphthalene –disulfonic acid (118-33-2) been reviewed to determine the toxic nature of target chemical 6 amino-1,3 naphthalene –disulfonic acid. The studies are as mentioned below:

In the key study study for the chemical from chemosphere ,Toxic effects of test substance 6 amino-1,3 naphthalene –disulfonic acid was determined on various fish species like Leuciscus idus, Brachydanio rerio, Salmo gaidneri, Pimephales promelas, Lepomis macrochirus, Poecilia reticulate for 96 hrs in static condition and in fresh water. During experiment the lethal concentration (LC0) value was1000 mg/l. Thus based on the above experimental result the test substance6 amino-1,3 naphthalene –disulfonic acid was nontoxic to fishes and thus not consider for the further classification as per the CLP criteria.

 

Similarly in the another supporting study for 6 amino-1,3 naphthalene –disulfonic acid from IUCLID dataset 2000. Toxic effects of test substance6 amino-1, 3 naphthalene –disulfonic acid was determined on Leuciscus idus for 96 hrs in static condition and in fresh water. During experiment the lethal concentration (LC0) value was ≥1000 mg/l. Thus based on the above experimental result the test substance6 amino-1, 3 naphthalene –disulfonic acid was nontoxic for fishes and thus not consider for the further classification as per the CLP criteria.

 

Based on the various experimental data for the target chemical 6 amino-1,3 naphthalene –disulfonic acid (118-33-2) from chemosphere and IUCLID dataset, the chemical was not toxic for fishes and thus not consider for the further classification as per the CLP criteria.

Short term toxicity to aquatic invertebrates:

Based on the various experimental data for the target chemical 6 amino-1,3 naphthalene –disulfonic acid (118-33-2) been reviewed to determine the toxic nature of target chemical 6 amino-1,3 naphthalene –disulfonic acid. The studies are as mentioned below:

In the first weight of evidence study from QSAR, 2017 for 1, 3-Naphthalenedisulfonic acid, 6-amino. Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on Daphnia magna was predicted for 118-33-2 (1, 3-Naphthalenedisulfonic acid, 6-amino). The EC50 value was estimated to be 138mg/l when1, 3-Naphthalenedisulfonic acid, 6-amino exposed to Daphnia magna for 48hrs.

 

Similarly in the another weight of evidence study for target chemical from EPIsuite 2017, Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted. On the basis of this program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be 720000mg/l for CAS name 6-aminonaphthalene-1, 3-disulfonic acid in 48 hrs. Based on this value it can be concluded that the substance is considered to be not toxic to aquatic environment and cannot be classified as per the criteria mentioned in CLP regulation.

Similar study for the RA chemical 1,2-benzisothiazol-3(2H)-one 1,1-dioxide (ABITEC s.r.o, 2016) which support the classification. Determination of the inhibition of the mobility of daphnids was carried out with the substance 1,2-benzisothiazol-3(2H)-one 1,1-dioxide according to OECD Guideline 202.The test substance was tested at the concentration 100 mg/L. 0% Daphnia were immobilized at the end of the test. The median effective concentration (EC50) for the test substance, 1,2-benzisothiazol-3(2H)-one 1,1-dioxide, in Daphnia magna was determined to be > 100 mg/L for immobilization effects. This value indicates that the substance is not likely to be hazardous to aquatic invertebrates as per the CLP criteria.

 

By considering another report for 1,2-benzisothiazol-3(2H)-one 1,1-dioxide (81-07-2), international environment journal. In terms of toxicity saccharin does not showed any statistically significant adverse effects in test concentrations up to 1000 mg L−1 (P > 0.05). Based on the lack of effects on all endpoints evaluated, the No Observed Effect Level Concentration (NOEC) can be given as 1000 mg L−1 and the Lowest Observed Effect Concentration (LOEC) as >1000 mg L−1 for the target compound. And thus the chemical 1,2-benzisothiazol-3(2H)-one 1,1-dioxide was consider as not toxic to aquatic invertebrates.

 

 

Based on the various experimental data for the target chemical 6 amino-1,3 naphthalene –disulfonic acid (118-33-2) from QSAR and EPI suite, ABITEC report and environmental journal the chemical was not toxic for daphnia magna and thus not consider for the further classification as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

Based on the various experimental data for the target chemical 6 amino-1,3 naphthalene –disulfonic acid (118-33-2) have been reviewed to determine the toxic nature of target chemical 6 amino-1,3 naphthalene –disulfonic acid. The studies are as mentioned below:

In the first weight of evidence study from QSAR, 2017 for 1, 3-Naphthalenedisulfonic acid, 6-amino. Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on Desmodesmus subspicatus was predicted for 118-33-2 (1, 3-Naphthalenedisulfonic acid, 6-amino). The EC50 value was estimated to be 121.060mg/l when1, 3-Naphthalenedisulfonic acid, 6-amino exposed to Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) for 72hrs.

 

Similarly in the another weight of evidence study for target chemical from Danish QSAR 2017, Based on the prediction done using the three different models i.e, Battery, Leadscope and SciQSAR used within Danish QSAR database, the short term toxicity on green algae was predicted for test substance 1,3-Naphthalenedisulfonic acid, 6-amino (118 -33 -2). The average EC50 value was given by the third model i.e, Battery model. On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance 1,3-Naphthalenedisulfonic acid, 6-amino  is estimated to be 8934.211 mg/l for Pseudokirchneriella subcapitata during 72 hr exposure duration. Thus, on the basis of EC50 value,it can be concluded that the test chemical  1,3-Naphthalenedisulfonic acid, 6-amino can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria.

Similarly in the another study for read across chemical 1,2-benzisothiazol-3(2H)-one 1,1-dioxide, (81 -07 -2) sustainable support services, Europe. Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 1,2-benzisothiazol-3(2H)-one 1,1-dioxide according to OECD Guideline 2. The test substance was dissolved in DMSO and tested at the concentrations 0, 17, 30, 55, 100 and 185 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance, 1,2-benzisothiazol-3(2H)-one 1,1-dioxide, in Desmodesmus subspicatus was determined to be 113.2 mg/L.This value indicates that the substance is not likely to be hazardous to aquatic algae as per the CLP criteria.

 

In the fourth weight of evidence study for read across chemical 1,2-benzisothiazol-3(2H)-one 1,1-dioxide, (81 -07 -2) environmental journal, In terms of toxicity saccharin showed any statistically significant adverse effects in test concentrations up to 1000 mg L−1 (P > 0.05). Based on the lack of effects on all endpoints evaluated, the No Observed Effect Level Concentration (NOEC) can be given as 1000 mg L−1 and the Lowest Observed Effect Concentration (LOEC) as >1000 mg L−1 for the target compound. Thus based on the NOEC and LOEC 1,2-benzisothiazol-3(2H)-one 1,1-dioxide was nontoxic and can be consider as not classified as per the CLP classification criteria.

Based on the various experimental data for the target chemical 6 amino-1,3 naphthalene –disulfonic acid (118-33-2) from QSAR Danish, ABITEC report and journal, the chemical was not toxic for algae and thus not classified as per the CLP classification criteria.

Toxicity to microorganisms:

Based on the key study from chemosphere, Toxic effects of test substance 6 amino-1 ,3 naphthalene –disulfonic acid was determined on bacteriafor 24 hrs in static condition and in fresh water.During experiment the effect concentration (EC0) value was1000 mg/l. Based on the experimental data the effect concentration (EC0) of test substancesubstance 6 amino-1, 3 naphthalene –disulfonic acid for bacteria was 1000mg/l. And thus the chemical was non toxic and not classified as per the CLP classification criteria.

Based on the toxicity on aquatic fish, invertebrates, algae and microorganisms 6 amino-1 ,3 naphthalene –disulfonic acid

was nontoxic and can be consider as not classified as per the CLP classification criteria.