Methyl ricinoleate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

None.

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

The inoculum was collected in the secondary effluent of the municipal STEP of Versailles (78, France), one day prior to the experiment. The inoculum was kept under aeration. Before use, it was centrifuged at 4000 g for 20 minutes at 20°C. The pellet was resuspended in test medium. The prepared inocolum was then aerated before use. The final concentration of suspended solids under test conditions was 21 mg/L.

Duration of test (contact time):

28 d

Initial conc.:

248.5 other: mg O2 / L under test conditions, i.e. 100.8 mg of substance per L.

Based on:

ThOD

Remarks:

Theoretical chemical demand in oxygen determined for the whole substance according to NF T 90101 standard.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

The experimental setup was done according to OECD 301F guideline. The dispersing agents Synperonic P94 and P103 were used. The study was conducted with 100.8 mg of substance per liter of test medium. This corresponded to a chemical oxygen demand of 248.5 mg oxygen per liter of test medium. CO2 was trapped with KOH pellets. Oxygen consumption was monitored with a manometer throughout the experiment.

Reference substance:

benzoic acid, sodium salt

Preliminary study:

No preliminary study.

Test performance:

All validity criteria passed.

Key result

Parameter:

% degradation (O2 consumption)

Value:

78

Sampling time:

27 d

Details on results:

77% biodegradation of the substance was reached within 27 days. 10% biodegradation was reached within 1-2 days. 60% biodegradation was reached within the 10 following days. Therefore, the substance is considered readily biodegradable.

Results with reference substance:

Biodegradation of the reference substance was higher than 60% within 14 days.

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Methyl ricinoleate is readily biodegradable according to REACh regulation criteria.

Executive summary:

A GLP study following OECD 301F test guideline was carried out for methyl ricinoleate. The inoculum was collected in a non-adapted secondary effluent of the municipal station of Versailles (78, France). Suspended solids concentration in the test medium under testing conditions was 21 mg/L. The chemical demand of the test item was determined in order to convert the biological demand in oxygen during the study into percentage of biodegradation. 10% biodegradation was reached within 1-2 days. 60% biodegradation was reached within the 10 following days. 77% biodegradation of the substance was reached within 27 d. Therefore, the substance is considered readily biodegradable.

Description of key information

A GLP study following OECD 301F test guideline was carried out for methyl ricinoleate. The inoculum was collected in a non-adapted secondary effluent of the municipal station of Versailles (78, France). Suspended solids concentration in the test medium under testing conditions was 21 mg/L. The chemical demand of the test item was determined in order to convert the biological demand in oxygen during the study into percentage of biodegradation. 10% biodegradation was reached within 1-2 days. 60% biodegradation was reached within the 10 following days. 77% biodegradation of the substance was reached within 27 d. Therefore, the substance is considered readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable