2-methyl-m-phenylene diisocyanate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

Static 96 h toxicity tests were performed with one freshwater species Pimephales promelas to determine influenced to aquatic toxicities.

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):deionized water or acetone

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name:Fathead minnow
- Source:EPA Newtown Fish Toxicology Station, Cincinnati, Ohio
- Length at study initiation (length definition, mean, range and SD):3.2 to 4.2 cm
- Food type:ration of Tetra SM
- Frequency:twice daily

ACCLIMATION
- Acclimation period:4 days
- Acclimation conditions (same as test or not):The minnows were acclimated to the test dilution water quality and temperature over the first two days of a fourday acclimation period. Solution withdrawal and renewal twice daily accomplished the water quality transition and temperature was reduced from 25 ° to 22°C with the aid of a temperature bath-circulator. The fish were held for the last two days of acclimation at 22°C in 100% dilution water.
- Type and amount of food:ration of Tetra SM
- Feeding frequency:no food was given during the four-day period
- Health during acclimation (any mortality observed):The fish were observed for a minimum of 14 days (at least 10 days after any disease treatment) before being acclimated for testing.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

40-48 mg/l as CaCO3

Test temperature:

22°C

pH:

7.2-7.9

Dissolved oxygen:

No data

Salinity:

alkalinity, 30-35 mg/l as CaCO3

Nominal and measured concentrations:

5 nominal concentration was used.

Details on test conditions:

TEST SYSTEM
- Test vessel:distilled water bottles with the necks removed
- Type (delete if not applicable): open
- No. of organisms per vessel:10
- No. of vessels per concentration (replicates):2
- No. of vessels per control (replicates):2
- Biomass loading rate:The loading of organisms per liter of dilution water was less than 0.8g1-1 in all cases.

OTHER TEST CONDITIONS
- Adjustment of pH:no
- Photoperiod:16h light-8 h dark diurnal cycle

Reference substance (positive control):

not specified

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

195 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 143.8-278.6

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

172 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 118.1-250.4

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

164 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 108.8-240.4

Details on results:

During the 96 h test period, deaths were recorded and bodies removed when noticed. Per cent survival, dissolved oxygen, and temperature were determined in each aquaria every 24 h.

Reported statistics and error estimates:

LC50's and confidence intervals were determined by either probit analysis (Finney, 1971; Barr et al., 1976), moving average method, or the binomial test (Stephan, 1977), depending on the number of partial kills observed. Each confidence interval describes the distribution of sensitivities of the test organisms: it does not indicate the precision of the acute mortality test.

Sublethal observations / clinical signs:

Solvent and recovery efficiencies for the detection of test compound in aqueous mixture:

Compound	Solvent	No. of trials	Mean %recovery	Std.deviation
Toluene-2,4-diisocyanat e	Benzene	5	89.6	2.0

 

Validity criteria fulfilled:

yes

Conclusions:

Toluene-2,4-diisocyanate was not found to be hazardous to freshwater fish Pimephales promelas with lethal concentration (LC50)195 mg/L for 24 hr; 172 mg/L for 48 hr; 164 mg/L for exposure period 96 hrs.

Executive summary:

Static 96 h toxicity tests were performed with one freshwater species Pimephales promelas (Fathead minnow)to determine influenced to aquatic toxicities.

For analytical analysis Model 2100 gas chromatograph equipped with electron capture detector and column of 3~ SE-30 on 100/120 Vataport 30 was used.and deionized water or acetone used as a vehicle.LC50's and confidence intervals were determined by either probit analysis (Finney, 1971; Barr et al., 1976), moving average method, or the binomial test (Stephan, 1977), depending on the number of partial kills observed. Each confidence interval describes the distribution of sensitivities of the test organisms: it does not indicate the precision of the acute mortality test.

LC50's and confidence intervals were determined by either probit analysis (Finney, 1971; Barr et al., 1976), moving average method, or the binomial test (Stephan, 1977), depending on the number of partial kills observed. Each confidence interval describes the distribution of sensitivities of the test organisms: it does not indicate the precision of the acute mortality test.

From experimetal result ot is concluded that the Toluene-2,4-diisocyanate was not found to be hazardous to freshwater fish Pimephales promelas with lethal concentration (LC50)195 mg/L for 24 hr; 172 mg/L for 48 hr; 164 mg/L for exposure period 96 hrs.

Description of key information

Toluene-2,4-diisocyanate was not found to be hazardous to freshwater fish Pimephales promelas with lethal concentration (LC50)195 mg/L for 24 hr; 172 mg/L for 48 hr; 164 mg/L for exposure period 96 hrs.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

164 mg/L

Additional information

Static 96 h toxicity tests were performed with one freshwater species Pimephales promelas (Fathead minnow)to determine influenced to aquatic toxicities.

For analytical analysis Model 2100 gas chromatograph equipped with electron capture detector and column of 3~ SE-30 on 100/120 Vataport 30 was used.and deionized water or acetone used as a vehicle.LC50's and confidence intervals were determined by either probit analysis (Finney, 1971; Barr et al., 1976), moving average method, or the binomial test (Stephan, 1977), depending on the number of partial kills observed. Each confidence interval describes the distribution of sensitivities of the test organisms: it does not indicate the precision of the acute mortality test.

LC50's and confidence intervals were determined by either probit analysis (Finney, 1971; Barr et al., 1976), moving average method, or the binomial test (Stephan, 1977), depending on the number of partial kills observed. Each confidence interval describes the distribution of sensitivities of the test organisms: it does not indicate the precision of the acute mortality test.

From experimetal result ot is concluded that the Toluene-2,4-diisocyanate was not found to be hazardous to freshwater fish Pimephales promelas with lethal concentration (LC50)195 mg/L for 24 hr; 172 mg/L for 48 hr; 164 mg/L for exposure period 96 hrs.