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EC number: 285-550-1
CAS number: 85116-97-8
Given that the toxicity cannot be atributed to a single component
or mixture of components but to the test item as a whole, the results
were based on nominal loading rates only.
Table 1: Inhibition of Growth Rate and Yield in the Definitive Test
Nominal loading rate [mg/L]
Growth rate [cells/mL/hour]
[Increase in growth as compared to the control]
Table 2: Validity criteria for OECD 201 (2006)
Criterion from the guideline
Validity criterion fulfilled
The biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72-hour test period.
The cell concentration of the control cultures increased by a factor of 148 after 72 hours.
The mean coefficient of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3, for 72-hour tests) in the control cultures must not exceed 35%
The mean coefficient of variation for section-by-section specific growth rate for the control cultures was 10%.
The coefficient of variation of average specific growth rates during the whole test period in replicate control cultures must not exceed 7% in tests with Pseudokirchneriella subcapitata and Desmodesmus subspicatus. For other less frequently tested species, the value should not exceed 10%.
The coefficient of variation of average specific growth rate for the control cultures over the test period (0 – 72 h) was 3%.
No effects up to the limit of water solubility (OECD 201)
One experimental study is available investigating the toxicity of
Fatty acids, C16-18, esters with diethylene glycol (CAS 85116-97-8) to
freshwater algae. The study was performed according to OECD 201 (GLP;
Vryenhoef, 2015). Since the test substance is poorly soluble in water,
Water Accommodated Fractions (WAF) of 10, 20, 40, 80 and 100 mg/L
(nominal) were prepared. The nominal loading rates were analytically
verified by HPLC/MS analysis and resulted in concentrations 0.00452 -
0.0407 mg/L at study initiation and depleted below the limit of
quantification at the end of the study after 72 h.
After incubation, inhibition of growth of 2% was recorded at 10 mg/L, 4%
at 20 mg/L, 7% at 40 mg/L, 8% at 80 mg/L and 7% at 160 mg/L. Statistical
analysis resulted in significant difference between the control and the
treatment of 40 mg/L. Thus, the NOELR (72 h) was set to 20 mg/L based on
growth rate. However, the inhibition of growth was < 10% in all
treatments resulting in an ErL10 (72 h) > 160 mg/L. These effects are
not considered to be biologically relevant and the ErL10 was used for
the final assessment and classification purposes. This is in line with
OECD Guidance on statistical evaluation of ecotoxicological data (OECD,
2006) on the guidance on application of CLP criteria (ECHA, 2015).
In conclusion the test substance will not exhibit toxic effects to
aquatic algae up to the limit of water solubility.
OECD 2006: Series on Testing and Assessment Number 54, Current
approaches in the statistical analysis of ecotoxicity data: a guidance
to application. ENV/JM/MONO(2006)18
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