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EC number: 285-550-1
CAS number: 85116-97-8
The toxicity control attained 74% biodegradation after 14 d and 69%
biodegradation after 28 d, thereby confirming that the test item did not
exhibit an inhibitory effect on STP microorganisms.
Table 1: Percentage biodegradation values
* On day 28, 1 mL of concentrated HCl was added to each vessel to drive
off any inorganic carbonates formed. The vessels were resealed, aerated
overnight and the final samples taken from both absorber vessels on day
Table 2: Validity criteria for OECD 301 B.
Criterion from the guideline
Validity criterion fulfilled
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%.
The difference between the values for CO2 production at the end of the test for the replicate vessels was <20%.
Percentage degradation of the reference compound reached the pass level by day 14 (≥ 60%).
Sodium benzoate attained 85% biodegradation after 14 days.
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d.
The toxicity control attained 74% biodegradation after 14 days and 96% biodegradation after 28 days.
The IC content of the test substance suspension in the mineral medium at the beginning of the test must be less than 5% of the TC.
The IC content of the test suspension in the mineral medium at the start of the test was below 5% of the TC content.
The total CO2 evolution in the inoculum blank at the end of the test should not normally exceed 40 mg/L medium.
The total CO2 evolution in the inoculum control vessels on Day 28 was 36.88 mg/L.
Readily biodegradable: 95% after 28 d (CO2 evolution, OECD 301B)
One experimental study is available investigating the ready
biodegradability of Fatty acids, C16-18, esters with diethylene glycol
(CAS 85116-97-8). The study was conducted according to OECD 301B (GLP)
using non-adapted activated sludge as inoculum. 14.4 mg of the test
substance were used and incubated for 28 d. The CO2 produced was
absorbed in 0.05 M NaOH solution and analysed. On day 28 concentrated
hydrochloric acid was added to each vessel in order to drive off any
inorganic carbon formed. The final measurement was done on day 29 but
refers to the degradation of the substance after 28 d. After 28 d the
test substance was degraded to 95% (mean of two replicates). Thus, the
substance is concluded to be readily biodegradable according to the OECD
The toxicity control attained 74% biodegradation after 14 d confirming
that the test substance is not inhibitory to the test item.
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