N-[3-(dimethylamino)propyl]oleamide

Long-term toxicity to aquatic invertebrates

Description of key information

Read-across from amides rape-oil, N-[3-(dimethylamino) propyl]:
In a 21-day semi-static toxicity test with Daphnia magna, the 21-day NOEC value and LOEC value, based on reproduction and adult length were 0.048 mg/L  and 0.15 mg/L respectively. The EC10 based on reproduction was 0.07 mg/L. The EC10 based on length was 0.14 mg/L.

Additional information

A reproduction study on aquatic invertebrates with N-[3-(Dimethylamino)propyl]oleamide is not available. Consequently, read-across was applied using a characteristically similar compound, amides rape-oil, N-[3-(dimethylamino) propyl].
In oder to assess the toxicity of the test substance in an aquatic environment, a Daphnia magna reproduction test under semi static conditions was conducted in accordance with OECD Guideline No. 211 and EU method C.20. Some modifications to the guideline were applied. The test was carried out according to the bulk approach using enriched natural surface water with a low Dissolved Organic Carbon (DOC) and Total Suspended Solids (TSS) content, allowing a more environmentally realistic determination of the effects of the test chemical to be made.
Young Daphnia, aged less than 24 hours, were exposed to the nominal concentrations of 0.004, 0.015, 0.048, 0.15 and 0.5 mg test item/L over a time period of 21 days. Primary test criterion of toxicity used was reproductive capacity, expressed as the total number of neonates per adult daphnid alive at the end of the test. Other endpoints based on adult mortality, length and weight were calculated if possible.
Analytical determinations of the test solutions showed that approximately 80% of the concentrations could be detected in the stock solution and in the highest 3 concentrations. The recovery in the lower concentrations fell below 80%. Loss of the chemical at lower concentrations was considered a characteristic of the test chemical and was accepted as part of the bulk approach. It was concluded that the test organisms were fully exposed to the test chemical in keeping with the bulk approach and the nominal concentrations were used for calculations of the effect levels.
Based on the reproduction and length data of the surviving daphnia a toxic effect could be determined. The NOEC value and LOEC value, based on reproduction and adult length were 0.048 mg/L and 0.15 mg/L respectively. The EC10 based on reproduction was calculated as 0.07 mg/L (confidence limits could not be calculated). The EC10 based on length was calculated as 0.14 mg/L (confidence limits could not be calculated). The dry weight endpoints was considered unreliable due to the inaccuracy involved in weighing the low number of surviving daphnids. The adult mortality endpoint was not considered reliable due to physical effects being the most likely cause of the effects.
The following validity criteria were fulfilled:
Mortality in the control did not exceed 20% during the test period.
The average number of juveniles per parent in the control was 61.