Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-825-2 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
E99434 is biodegraded 18% at day 28 in the Closed Bottle test. In the prolonged Closed Bottle test the test substance is biodegraded 30% at day 60. E99434 should therefore be classified as inherently biodegradable, this test indicating an unequivocal evidence of biodegradation (>20%).
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
Additional information
In order to assess the biotic degradation, a ready biodegradability test (OECD 301D) was performed which allows the biodegradability to be measured in an aerobic aqueous medium. The ready biodegradability was determined in the Closed Bottle test performed according to slightly modified OECD, EU and ISO Test Guidelines, and in compliance with the OECD principles of Good Laboratory Practice. The test was prolonged because the pass level was not reached at day 28.
E99434 did not cause a reduction in the endogenous respiration.
E99434 is biodegraded 18% at Day 28 in the Closed Bottle test. In the prolonged Closed Bottle test the test substance is biodegraded 30% at Day 60. E99434 should therefore be classified as inherently biodegradable, this test indicating an unequivocal evidence of biodegradation (>20%).
To determine the true biodegradability a method to increase the bioavailability was used (van Ginkel et al, 2008). Although the bioavailability is increased by the method used, poor water solubility is still affecting biodegradation kinetics because slow desorption and dissolution rates of test substance present at high concentrations in the ready biodegradability test. The biodegradation curves of substances with limited bioavailability are linear instead of the anticipated S-shape. The partial degradation (i.e. 30%) is therefore caused by the limited bioavailability. It should be noted that under environmental conditions E99434 will be present in the environment at concentrations in the range of µg/L or lower. Under those conditions, complete degradation is expected within a shorter time period.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
