Acetoacetanilide

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed according to OECD-Guideline and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Acetoacetanilide

EC Number:

202-996-4

EC Name:

Acetoacetanilide

Cas Number:

102-01-2

Molecular formula:

C10H11NO2

IUPAC Name:

3-oxo-N-phenylbutanamide

Details on test material:

Acetoacetanilide - dry; expiration date 05/19/97; storage conditions: 75°C;

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The nominal test concentrations were prepared by dilution of stock solution (test item in fresh water algal medium).

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

- Origin: UTEX- The Culture Collection of Alga e at the University of Texas.
- Maintained at culture medium at Wildlife Int. Ltd. Easton, Maryland for at least two weeks.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

24.4 - 25.2°C

pH:

7.5 - 9.1

Nominal and measured concentrations:

nominal: 0, 45, 90, 180, 360, 720 mg/L

Details on test conditions:

- Medium is prepared following the ASTM Standard Guide 1218-90-E. Standard Guide for conducting static 96-hour toxicity tests with microalgae. August 1990.
- Test is done with triplicates for each concentration

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

180 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

318 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

- Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: no
- Colour differences: no

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the test condions the EC50 (algae growth rate) was determined as 318 mg/L for Acetoacetanilide

Executive summary:

The result of this test gives no reason for classification