Acetoacetanilide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Acetoacetanilide

EC Number:

202-996-4

EC Name:

Acetoacetanilide

Cas Number:

102-01-2

Molecular formula:

C10H11NO2

IUPAC Name:

3-oxo-N-phenylbutanamide

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: after 48 and 96 h

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Source: HOECHST own breed
- Length at study initiation (length definition, mean, range and SD): 2.7 - 3.1 cm, mw 2.9


ACCLIMATION
- Acclimation period: 14 d
- Acclimation conditions (same as test or not): as test conditions
- Type and amount of food:
- Feeding frequency: 2 x a day, ad libitum
- Health during acclimation (any mortality observed): no data

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

21.7 - 22.2°C

pH:

7.4 - 8.3

Dissolved oxygen:

6.9 - 8.8 mg/l

Nominal and measured concentrations:

nominal: 0 / 180 / 250 / 350 / 500 mg/l
measured: 0 / 177 / 235 / 242 / 332 mg/l

Details on test conditions:

reconstituted water acc. ISO/DIN 7346/1

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

242 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 242 - < 332 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: 0
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: in the higher concentrations (242 and 332 mg/l) small amounts of precipitates were found at the bottom of the tanks

Results with reference substance (positive control):

no data

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The LC50 after 48 and 96 hours is > 242 mg/L and the LC50 is betwen 242 and 332 mg/L. The substance is not considered to be toxic for fish.