2-ethylhexyl chloroformate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

yes

Remarks:

smaller application volume

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-ethylhexyl chloroformate

EC Number:

246-278-9

EC Name:

2-ethylhexyl chloroformate

Cas Number:

24468-13-1

Molecular formula:

C9H17ClO2

IUPAC Name:

2-ethylhexyl carbonochloridate

Details on test material:

- Name of test material (as cited in study report): 2-Ethylhexylchlorformiat (2-ethylhexylchloroformate)
- Physical state: liquid
- Analytical purity: 99%
- Impurities (identity and concentrations): phosgene and Di-2ethylhexylcarbonate
- Storage condition of test material: dry, cool, in glas containers
- Sability under storage conditions > 2 years

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: Gaukler, Offenbach, Germany
- Weight at study initiation: 2.88 kg (F); 2,76 kg (m)
- Housing: single
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
not reported

Test system

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 µl

Duration of treatment / exposure:

eye s remained unwashed

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

2

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no washing

SCORING SYSTEM:
Descriptive scoring system comparable to Daize

TOOL USED TO ASSESS SCORE: fluorescein

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

animal #1

Remarks:

mean

Time point:

24/48/72 h

Score:

0.33

Max. score:

1

Reversibility:

fully reversible within: 2 days

Results 2

Irritation parameter:

conjunctivae score

Basis:

animal #1

Remarks:

mean

Time point:

24/48/72 h

Score:

1.33

Max. score:

2

Reversibility:

fully reversible within: 4 days

Results 3

Irritation parameter:

cornea opacity score

Basis:

animal #2

Remarks:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

0

Reversibility:

not specified

Results 4

Irritation parameter:

conjunctivae score

Basis:

animal #2

Remarks:

mean

Time point:

24/48/72 h

Score:

1

Max. score:

1

Reversibility:

fully reversible within:

Remarks:

4 days

Results 5

Irritation parameter:

iris score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

0

Reversibility:

not specified

Results 6

Irritation parameter:

chemosis score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

0

Reversibility:

not specified

Irritant / corrosive response data:

The results indicate a potential of eye irritation. As dose volume do not comply with the test guidelines, the hazard cannot be assessed definitly in comparisson to OECD TG405.

Any other information on results incl. tables

Descriptive scores of the raw data have been converted to Draize numerical scores.

Animal	Reading	Opacity	Redness
1	24h	1	2
1	48 h	0	1
1	72 h	0	1
1	4 d	0	0
mean	24 - 72 h	0,33	1,33
2	24h	0	1
2	48 h	0	1
2	72 h	0	1
2	4 d	0	0
mean	24 - 72 h	0	1