Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-824-2 | CAS number: 74-95-3
Endpoint summary
Administrative data
Description of key information
Key value for chemical safety assessment
Skin sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
- Additional information:
Following a preliminary screening test in which no clinical signs of toxicity were noted at a concentration of 100% dibromomethane, this concentration was selected as the highest dose investigated in order to assess the skin sensitisation potential of the test material in the CBA/Ca strain mice.
Three groups, each of five animals, were treated by topical application to the dorsal surface of the ear with 25 µl undiluted test material (99.5% purity, dibromomethane) or the test material as a solution in acetone/olive oil 4:1 at concentrations of 50% or 25% v/v. A further group of five animals was treated with acetone/olive oil 4:1 alone.
Results: The Stimulation Index (SI) of the vehicle control group were 1.39, 0.63 and 0.70 for all concentrations of the test material investigated (100%, 50% & 25%, respectively). The positive control group (a concentration of 15% of α-hexylcinnamaldehyde in 4:1 Acetone/Olive oil) showed an acceptable response for a valid study with an SI of 4.10. Hence, it was concluded that the DBM is not skin sensitizer.
Migrated from Short description of key information:
Test/Control < 3 for all concentrations of the test material investigated (100%, 50% & 25%). The stimulation indexes (SI's) were 1.39, 0.63 and 0.70 respectively. The test material is a non sensitiser.
The positive control group (a concentration of 15% of alpha-hexylcinnamaldehyde in 4:1 Acetone/Olive oil) showed an acceptable response for a valid study with an SI of 4.10.
Justification for selection of skin sensitisation endpoint:
only study available.
Respiratory sensitisation
Endpoint conclusion
- Additional information:
Not sensitizing
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
