Cyclohexylethylcarbamoyl chloride

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study according to EU /OECD guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Principles of method if other than guideline:

Two (technical) deviations from the study plan had no influence in the results of the study:
1. Dosing was altered depending on the actual development of mortality.
2. The post exposure observation period was prolonged to 21 days.

GLP compliance:

yes (incl. QA statement)

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Cyclohexylethylcarbamoyl chloride

EC Number:

263-753-6

EC Name:

Cyclohexylethylcarbamoyl chloride

Cas Number:

62899-75-6

Molecular formula:

C9H16ClNO

IUPAC Name:

N-cyclohexyl-N-ethylcarbamoyl chloride

Test material form:

other: liquid

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

nose only

Vehicle:

air

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

580 mg/m3
880 mg/m3
1390 mg/m3

No. of animals per sex per dose:

5 male, 5 female

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

880 mg/m³ air

Based on:

test mat.

Exp. duration:

4 h

Mortality:

In all groups mortality occurred 7 - 9 days after exposure.

Clinical signs:

other: Main signs of clinical intoxication in all groups were lethargy, dyspnoe, partly combined with breathing sounds and salivation. Additionally in the medium and high dose group sneezing occurred and in the high dose group tremor was observed for about 2 day

Body weight:

Body weight decreased up to day 3 in the low dose group and up to day 7 in the medium and high dose group. After this time body weight increased but did not reach normal values up to day 21.

Gross pathology:

In the test substance treated groups of this study during necropsy all but one lungs of the rats were bloated to a dose dependent degree.
In the low dose group 8 lungs were bloated slightly and 1 moderately. 1 lung had additionally small white areas, up to 1 mm in diameter and the only lung which was not bloated had 1 grey glassy area, about 1 mm in diameter. The liver of one female had 2 deformed lobes, a 3rd lobe had one white area, about 2 mm in diameter. In the medium dose group 3 of the lungs were bloated slightly, 5 moderate and 2 severe. 1 lung was pinkish coloured, another one showed multiple bright-red areas and a third one diffuse brownred areas. In 2 male rats the lung associated lymph nodes were enlarged. In one male and female each thymus had dark-red areas up to 1 mm in diameter. In the high dose group 1 lung was bloated moderately and the other 9 severe. 4 of the lungs were more or less pinkish, one had dark-red areas up to 5 mm in diameter. The lung associated lymph nodes were dark-red in one male and enlarged in an other male. In one female thymus was edematous.

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category III

Remarks:

Migrated information Criteria used for interpretation of results: EU