Octasodium 2-(8-(4-chloro-6-(3-((4-chloro-6-(3,6-disulfonato-2-(1,5-disulfonatonaphthalen-2-ylazo)-1-hydroxynaphthalen-8-ylamino)-1,3,5-triazin-2-yl)aminomethyl)phenylamino)-1,3,5-triazin-2-ylamino)-3,6-disulfonato-1-hydroxynaphthalen-2-ylazo)naphthalene-1,5-disulfonate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 December 1991 to 12 December 1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted to recognised EU test guideline in compliance with GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

84/449/EEC

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Octasodium 2-(8-(4-chloro-6-(3-((4-chloro-6-(3,6-disulfonato-2-(1,5-disulfonatonaphthalen-2-ylazo)-1-hydroxynaphthalen-8-ylamino)-1,3,5-triazin-2-yl)aminomethyl)phenylamino)-1,3,5-triazin-2-ylamino)-3,6-disulfonato-1-hydroxynaphthalen-2-ylazo)naphthalene-1,5-disulfonate

EC Number:

413-550-5

EC Name:

Octasodium 2-(8-(4-chloro-6-(3-((4-chloro-6-(3,6-disulfonato-2-(1,5-disulfonatonaphthalen-2-ylazo)-1-hydroxynaphthalen-8-ylamino)-1,3,5-triazin-2-yl)aminomethyl)phenylamino)-1,3,5-triazin-2-ylamino)-3,6-disulfonato-1-hydroxynaphthalen-2-ylazo)naphthalene-1,5-disulfonate

Cas Number:

142068-96-0

Molecular formula:

Hill formula: C53H28Cl2N14Na8O26S8

IUPAC Name:

octasodium 5-[(4-chloro-6-{[4-({[4-chloro-6-({7-[2-(1,5-disulfonatonaphthalen-2-yl)diazen-1-yl]-8-hydroxy-3,6-disulfonatonaphthalen-1-yl}amino)-1,3,5-triazin-2-yl]amino}methyl)phenyl]amino}-1,3,5-triazin-2-yl)amino]-3-[2-(1,5-disulfonatonaphthalen-2-yl)diazen-1-yl]-4-hydroxynaphthalene-2,7-disulfonate

Details on test material:

- Name of test material (as cited in study report): H112339 (reference as sample V723)
- Lot/batch No.: NBY 405/74
- Storage condition of test material: darkness at an ambient temperature

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable.

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

The concentration of V723 in the test solutions was measured at the start (excess solution) and end of the test. At the end of the test one replicate of each test concentration was sampled.

Test solutions

Details on test solutions:

A solution of 112 mg V723/litre was prepared by addition of V723 to the dilution medium.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The test organism was the freshwater crustacean Daphnia magna Straus, obtained from continuous laboratory cultures.
The stock cultures of Daphnia were maintained in a reconstituted water medium, identical to the test dilution water, at a temperature of 20 ± 2°C, and a photoperiod of 16 hours light: 8 hours dark.
The cultures were fed a defined diet of algae Chlorella vulgaris and yeast.
Culture conditions were such that the Daphnia reproduction was by diploid parthenogenesis.
Daphnia <24 hours old were used for testing.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

No post exposure observation

Test conditions

Hardness:

Hardness (total as CaCO3) 179 mg/l

Test temperature:

The temperatures recorded at 0, 24 and 48 hours were 19.8, 19.5 and 19.8°C respectively

pH:

8.4-8.5

Dissolved oxygen:

9.0 - 9.5 mg/l

Salinity:

Not applicable.

Nominal and measured concentrations:

A solution of 112 mg V723/litre was prepared by addition of V723 to the dilution medium. Information supplied with the test sample V723 indicated that it contained 10.5% of water and test concentrations were adjusted accordingly to account for a nominal test concentration of 100 mg/l.

Details on test conditions:

Borosilicate glass beakers were used as test vessels, with four replicates per exposure concentration, each containing 200 ml of test solution.
When the test solutions were at test temperature, five Daphnia were added to each vessel in <2.0 ml of dilution water, giving a total of 20 Daphnia per concentration.
The temperature was maintained at 20 ± 1°C and a photoperiod of 16 hours light: 8 hours dark was provided.
The Daphnia were not fed during the course of the test.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The 24 and 48 hour median effective concentrations of Substance HI 12339 to the freshwater crustacean Daphnia magna (<24 hours old) were determined under static test conditions at a temperature of 20 ± 1°C.
The median effective concentration (EC50) is defined as the concentration, calculated from the data obtained, resulting in immobilisation of 50% of the Daphnia in the time period specified.
The values obtained were:

EC50 (mg/l)
24 hour >100
48 hour >100

The values obtained were based on nominal concentrations of Substance HI 12339.
The overall mean of the measured concentrations of the test material as determined at the start and finish of the test was 90% of the nominal value.

Results with reference substance (positive control):

Not applicable.

Reported statistics and error estimates:

Not reported.

Any other information on results incl. tables

Daphnia response

Time	Nominal	Number affected per				Total	Total	%
(hours)	concn		replicate			number	number	affected
	(mg/1)	A	B	C	D	tested	affected
24	Control	0	0	0	0	20	0	0
	112	0	0	0	0	20	0	0
48	Control	0	0	0	0	20	0	0
	112	0	0	0	0	20	0	0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

As no effect was found, the 24 and 48 hr EC50 values were >112 mg V723/1 or >100 mg Substance HI 12339/1.
These values were calculated on the basis of the nominal concentration of V723.

Executive summary:

Study conducted to EU test guidance 84/449/EEC part C2 in compliance with GLP.

No effect was found at a concentration of 100mg/l after 48 hours. EC50> 100mg/l. The substance is not considered to pose a hazard to Daphnia.