Reaction mass of N1,N1'-(methanediyldibenzene-4,1 -diyl)diaryldiamine and 2-{4-[(4-aminophenyl)amino]benzyl}-N4-(4-{4-[(4-aminophenyl)amino]benzyl}phenyl)aryldiamine

Endpoint summary

Description of key information

Short-term toxicity to earthworm

The highest concentration of test substance causing no mortality at 7 days was 640 mg/kg dw soil (7d-LC0) and the LC50 (7d) value was reported as 1241 mg/kg dw soil. The highest concentration of substance causing no mortality at 14 days was 640 mg/kg dw soil (14d-LC0) and the LC50 (14d) value was reported as 884 mg/kg dw soil (HJ/T 153 -2004, OECD 207 and EU Method C.8).

Toxicity to plants

Under valid conditions, the phytotoxicity of the test substance to ten terrestrial plant species was conducted according to “The guidelines for the testing of chemicals” (HJ/T 153-2004). The LOEC values for germination were determined to be > 1000 mg/L in all ten species, NOEC values for germination were 1000 mg/L in all ten species and EC50 values for germination were > 1000 mg/L in all ten species. With respect to root elongation, LOEC values were determined to be > 1000 mg/L for all species apart from rice, soybean and radish where the LOEC was reported as 1000 mg/L. The NOEC for root elongation was 1000 mg/L in all species apart from rice, soybean and radish where the NOEC was reported as 100 mg/L. The EC50 value for root elongation was > 1000 mg/L in all ten species. No definitive testing was performed because the inhibition rates for root elongation were < 50 %.

Additional information

Short-term toxicity to earthworm

GUIDELINE

The test was conducted to determine the acute toxicity of the test substance to earthworm according to “The guidelines for the testing of chemicals” (HJ/T 153-2004) and “The Guidelines for the Testing of Chemicals-Effects on Biotic Systems” (the 2nd edition, Beijing: China Environment Press, 2013) with reference to Procedure 207 of the “Guidelines for Testing of Chemicals” of the OECD: “Earthworm, Acute Toxicity Tests” (1984) and EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test).

 

METHODS

The study was designed to determine the acute toxicity of test substances to earthworm at target concentrations of 0, 640, 800, 1000, 1250 and 1500 mg/kg dw soil. In this test, earthworms were exposed in samples of the artificial soil to which a range of concentrations of the test substance had been applied. Mortality was assessed 7 days and 14 days after application. The study comprised a range-finding test followed by a definitive test to determine the median lethal concentration (LC50) of the test substance.The moisture content of artificial soils was controlled in the range 26 to 35 % and pH 5.61 to 6.47 were used during the test. Temperature was maintained at 20.6 to 22.3 °C, humidity was maintained at 80 to 85 % and continuous light with intensity of 400 to 800 lux was supplied.

 

RESULTS

The highest concentration of test substance causing no mortality at 7 days was 640 mg/kg dw soil (7d-LC0) and the LC50 (7d) value was reported as 1241 mg/kg dw soil. The highest concentration of substance causing no mortality at 14 days was 640 mg/kg dw soil (14d-LC0) and the LC50 (14d) value was reported as 884 mg/kg dw soil.

Toxicity to plants

GUIDELINE

Under valid conditions, the phytotoxicity of the test substance to ten terrestrial plant species was conducted according to “The guidelines for the testing of chemicals” (HJ/T 153-2004).

 

METHODS

Ten plant species includingBrassica chinensis L. (Cabbage), Lactuca sativa (Lettuce), Oryza sativa L. (Rice), Brassica oleracea (Kohlrabi), Cucumis sativus (Cucumber), Zea mays (Corn), Sesamum indicum (Sesame), Lycopersicon esculentum (Tomato), Glycine max (Soybean), Raphanus sativus (Radish) (15 seeds/group/species) were exposed to 10, 100 and 1000 mg/L of the test substance. A blank control and a solvent control without the test substance were run at the same time. During the whole test period, the pH values of the freshly prepared control and Test Media were between 7.56 and 7.71, and the temperature in the germination facility was maintained in the range of 24 to 25 °C. At the end of the test, more than 65 % of the seeds in blank control and solvent control germinated, and the roots developed were longer than 20 mm. The study met the acceptability criteria prescribed by the protocol and the test was considered valid.

 

RESULTS
The initial concentration of test item in the test solution at the start of the test was determined by UPLC-PDA. According to the test result, the analysed concentration of 10 mg/L, 100 mg/L and 1000 mg/L were 9.31 mg/L, 95.5 mg/L and 903 mg/L respectively. There was no significant phytotoxicity observed in the range-finding test for Brassica chinensis L. (Cabbage), Lactuca sativa (lettuce), Brassica oleracea (Kohlrabi), Cucumis sativus (cucumber), Zea mays (corn), Sesamum indicum (sesame), Lycopersicon esculentum (tomato) (p >0.05). Although there were significant phytotoxicity observed in the range-finding test forOryza sativaL. (Rice), Glycine max (soybean) and Raphanus sativus (Radish) (p <0.05), the inhibition rates for root elongation were < 50%. Therefore, no definitive testing was carried out. The results showed that under valid test conditions, the LOEC, NOEC and EC50 for all ten plant species tested were as shown in the table below.

 

Seed	Germination LOEC (mg/L)	Germination NOEC (mg/L)	Germination EC50 (mg/L)	Root elongation LOEC (mg/L)	Root elongation NOEC (mg/L)	Root elongation EC50 (mg/L)
Cabbage	> 1000	1000	> 1000	> 1000	1000	> 1000
Lettuce	> 1000	1000	> 1000	> 1000	1000	> 1000
Rice	> 1000	1000	> 1000	1000	100	> 1000
Kohlrabi	> 1000	1000	> 1000	> 1000	1000	> 1000
Cucumber	> 1000	1000	> 1000	> 1000	1000	> 1000
Corn	> 1000	1000	> 1000	> 1000	1000	> 1000
Sesame	> 1000	1000	> 1000	> 1000	1000	> 1000
Tomato	> 1000	1000	> 1000	> 1000	1000	> 1000
Soybean	> 1000	1000	> 1000	1000	100	> 1000
Radish	> 1000	1000	> 1000	1000	100	> 1000

The measured concentration for a nominal concentration of 1000 mg/L was 903 mg/L

The measured concentration for a nominal concentration of 100 mg/L was 95.5 mg/L