2,4-dichloro-1,3-dinitro-5-(trifluoromethyl)benzene

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2004

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.4 (Acute Toxicity: Dermal Irritation / Corrosion)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,4-dichloro-1,3-dinitro-5-(trifluoromethyl)benzene

EC Number:

249-420-8

EC Name:

2,4-dichloro-1,3-dinitro-5-(trifluoromethyl)benzene

Cas Number:

29091-09-6

Molecular formula:

C7HCl2F3N2O4

IUPAC Name:

2,4-dichloro-1,3-dinitro-5-(trifluoromethyl)benzene

Details on test material:

Identification: 2,4 dichloro 3,5 dinitro benzotrifluoride
Description: Straw yellow powder
Purity: 96.90%
Stability of test item: Stable under storage conditions
Storage conditions: at room temperature, light protected

Test animals

Species:

rabbit

Strain:

New Zealand White

Test system

Type of coverage:

semiocclusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

yes

Duration of treatment / exposure:

4 hours

Number of animals:

3 (animals of both sexes were used)

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 1 hour

Score:

4

Max. score:

4

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 24 hours

Score:

4

Max. score:

4

Results 3

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 48 hours

Score:

2.3

Max. score:

3

Results 4

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 72 hours

Score:

2.3

Max. score:

3

Results 5

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 72 hours

Score:

2

Max. score:

2

Any other information on results incl. tables

Irritation

The mean score was calculated across 3 scoring times for each animal for erythema/eschar grades and for oedema grades, separately. The mean erythema/eschar score of the three animals was 2.00 for all three animals and the mean oedema score was 2.67, 3.33 and 2.67, respectively.

Well-defined erythema was observed in all animals from the 1hour reading up to 72hours after treatment. Slight erythema was noted in all animals at the 7day examination and persisted in one animal up to 10days after treatment.

Blanching was observed in one animal 1hour after treatment.

Severe oedema was visible in all animals from the 1 to the 24 hour reading. Slight to moderate oedema was still visible in all animals at the 48 and 72 hour reading.

Scaling was noted in all animals 10 and 14 days after treatment, the end of the observation period for all animals.

Corrosion

Neither alterations of the treated skin were observed nor were corrosive effects evident on the skin.

Coloration

Slight yellow staining produced by the test item of the treated skin was observed in all animals from the 24hour reading up to 14days after treatment, the end of the observation period for all animals.

Applicant's summary and conclusion

Interpretation of results:

irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The test item is considered to be “irritating” to rabbit skin and has to be labeled as R38.