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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Description of key information
Key value for chemical safety assessment
Skin sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
- Additional information:
The aim of this study was to determine the skin sensitisation potential of Golden Yellow Continuous following dermal exposure.
Based on results of the Preliminary Compatibility Test and on recommendations of the OECD Test Guideline 429, the test item was dissolved in Dimethyl-sulphoxide (DMSO). The maximum attainable concentration based on solubility of the test item in DMSO was 10%.
A Preliminary Irritation/Toxicity Test was performed with the test item at concentrations of 10% and 5% in the selected vehicle. According to this test, the applicability and biocompatibility of the test item to the animals’ ears (2 female CBA/J@Rj mice/dose) at the maximum concentration of 10% was acceptable.
In the main assay twenty female CBA/J@Rj mice were allocated to five groups of four animals each:
- three groups received the appropriate formulation of Golden Yellow Continuous at concentrations of 10%, 5% and 2.5%,
- the negative control group received the vehicle (DMSO),
- the positive control group received 25% alpha-Hexylcinnamaldehyde (HCA) in DMSO.
The test item solutions were applied to the dorsal surface of both ears of the mice (25 µL/ear) for three consecutive days (Days 1, 2, and 3). There was no treatment on Days 4, 5, and 6. On Day 6, the cell proliferation in the local lymph nodes was measured by incorporation of tritiated methyl thymidine (3HTdR) and the values obtained were used to calculate stimulation indices (SI).
No mortality or systemic clinical signs were observed during the study. No treatment related effects were observed on animal body weights in any of the treated groups. No cutaneous reactions were observed at the site of the treatment in any of the treated groups.
No significant lymphoproliferative response (SI ³ 3) of Golden Yellow Continuous was observed at the concentrations applied compared to the negative control. The stimulation index values of the test item were 1.6, 1.7, and 2.6 at treatment concentrations of 10%, 5%, and 2.5%, respectively. No conventional dose-response to treatment was observed.
A significant lymphoproliferative response (stimulation index value of 6.6) was noted for the positive control chemical HCA. This result confirmed the validity of the assay.
In conclusion, under the conditions of the present assay Golden Yellow Continuous, tested in a suitable vehicle, proved to have no sensitization potential (non-sensitizer) in the Local Lymph Node Assay.
Migrated from Short description of key information:
No sensitizing properties observed
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