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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Not readily or inherently biodegradable

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

A study was performed to assess the ready biodegradability of Golden Yellow Continuous.

The study was conducted in accordance with the OECD Guideline for Testing of chemicals No. 301 F 'Manometric respirometry Test' (adopted July 17, 1992).

A solution of Golden Yellow Continuous in a mineral medium was inoculated and incubated for 28 d under aerobic conditions. During this period, degradation was followed by BOD determinations.

Test item showed 5%degradation after 28 days

Therefore, Golden Yellow Continuous is considered to be "Not Readily Biodegradable".

The control substance monoethylene glycol showed 82%degradation after 14 days, proving the validity of the test system.

In addition, a study for inherent biodegradability ofGolden Yellow Continuouswas performed according to the OECD Guidelines for the Testing of Chemicals, Inherent Biodegradability: Modified MITI Test II.: OECD, Adopted 12 May 1981.

As the test substance was difficult soluble in water, 15.0 mg test substance was added to either deionized water (abiotic control) or medium, with transonic treatment for 15 min to achieve a good dispersion, and the solution was then directly transferred to the testbottles.

At the start of the test, NO2--N and NO3--N concentrations of the test suspensions were <0.03mg/L and 0.05mg/L, respectively. NO2--N and NO3--N concentrations of the inoculum blank were <0.03mg/L and 0.04mg/L respectively. At the end of the test, NO3--N concentrations of the test suspensions and inoculum blank were 5.99 mg/L, 5.83 mg/L, 6.04 mg/L (3 replicates) and 6.56 mg/L, NO2--N concentrations of the test suspensions and the inoculum blank were <0.03mg/L. The results revealed that there was no nitrification in test suspensions.

Results of recovery tests showed that the percent recovery of the test substance from water + test substance was 81.5% and the recovery from test medium + inoculum + test substance was 82.8% at start of the study.

At the end of the test, the residual content of test substance of abiotic control and the three test suspensions were 13.2 mg and 12.3 mg, 12.3 mg and 12.2 mg (3 replicates), respectively. Considering the recovery rate, the reduction of the test substance in abiotic control might be due to the loss during extraction process of test substance but not non-biodegradation.

The percentage primary biodegradation of test suspensions were 6.2%, 6.5%, and 7.5% (3 replicates).

The percentage biodegradation at the end of the test by BOD was 0%.