Octasodium 2,2'-[1,4-phenylenebis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino(1-hydroxy-3,6-disulphonatonaphthalene-2,8-diyl)azo]]bisnaphthalene-1,5-disulphonate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

2008

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

yes

Remarks:

The algal growth inhibition test was modified from Dutka & Bitton (1986), Toxicity Testing Using Microorganisms, Vol. 2, CRC Press, Inc, Florida, pp. 79-100. Total exposure duration: 96 h

Principles of method if other than guideline:

The algal growth inhibition test was modified from Dutka & Bitton (1986), Toxicity Testing Using Microorganisms, Vol. 2, CRC Press, Inc, Florida, pp. 79-100.
Total exposure duration: 96 h

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Octasodium 2,2'-[1,4-phenylenebis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino(1-hydroxy-3,6-disulphonatonaphthalene-2,8-diyl)azo]]bisnaphthalene-1,5-disulphonate

EC Number:

275-108-6

EC Name:

Octasodium 2,2'-[1,4-phenylenebis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino(1-hydroxy-3,6-disulphonatonaphthalene-2,8-diyl)azo]]bisnaphthalene-1,5-disulphonate

Cas Number:

71002-20-5

Molecular formula:

C52H26Cl2N14Na8O26S8

IUPAC Name:

octasodium 3-({4-chloro-6-[(4-{[4-chloro-6-({8-[(1E)-2-(1,5-disulfonatonaphthalen-2-yl)diazen-1-yl]-1-hydroxy-3,6-disulfonatonaphthalen-2-yl}amino)-1,3,5-triazin-2-yl]amino}phenyl)amino]-1,3,5-triazin-2-yl}amino)-5-[(1E)-2-(1,5-disulfonatonaphthalen-2-yl)diazen-1-yl]-4-hydroxynaphthalene-2,7-disulfonate

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Details on test solutions:

TEST WATER:
The test water adapted from Dutka & Bitton (1986) was used as a culture medium for dye toxicity testing to algae.

Test organisms

Test organisms (species):

Chlorella sp.

Details on test organisms:

The Chlorella sp. was obtained from cultured maintained at the Thailand Institute of Scientific and Technical Research. It was grown in 5 L glass jars containing growth medium to obtain the stock algal culture to be used for all experiments.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

4 d

Test conditions

Test temperature:

ambient temperature

Nominal and measured concentrations:

nominal concentrations: 50, 100, 150, 200, 250 mg/L

Reference substance (positive control):

yes

Remarks:

humic acid

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

95.55 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Any other information on results incl. tables

When consider the absorbance of Reactive Red 141, it is evident that Reactive Red 141 give the maximum magnitude of wavelength 544 nm. Hence, it could affect the light transmitted in the water, ultimately affecting the light absorption of Chlorophyll a in photosynthesis.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

In this toxicity test to algae (Chlorella sp.) an 96 hr-EC 50 value of 95.55 mg/L was obtained for Reactive Red 141.

Executive summary:

The algal growth inhibition test was modified from Dutka & Bitton (1986) and performed in 500 mL Erlenmeyer flask.

Algae were added into 100 mL of test water containing various concentrations of Reactive Red 141 (final concentration of 1 x 105 cells/mL). The flask was placed in an ambient temperature provided air and sealed with parafilm. Flasks were continuous illuminated (cool white fluorescent light approximately 120 ~J.E/s/m2 or 6,000 lux at water level. Determination of algal growth by measuring live cell count, Chlorophyll a, dry weight and absorbance at 720 nm (this wavelength is not interfered by the dyes) was done. The ECso for dyes toxicity to algae was estimated. The toxicity to algae was reported in term of 96-h EC50 which referred to the concentration of dye that inhibit the the growth of Chlorella sp. in 50% of the population after 96 h exposure. An 96 hr-EC 50 value of 95.55 mg/L was obtained for Reactive Red 141.

As Reactive Red 141 is a coloured substance, the effect of light absorption at standard algae growth inhibition tests has to be considered. Absorption of light at relevant wavelengths may cause an indirect effect on aquatic plant growth by inhibition of photosynthesis.