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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 22/04/1996 (initiation of the preliminary study) to 04/07/1996 (date of the report)
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study performed in accordance with the EU Directive 92/69/EEC C.2. As the test conditions are not fully described and no analytical monitoring has been performed, this study is considered as reliable with restrictions.

Data source

Reference
Title:
Unnamed
Year:
1996
Report date:
1996

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
not specified
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-hydroxy-4-(methylthio)butyronitrile
EC Number:
241-756-3
EC Name:
2-hydroxy-4-(methylthio)butyronitrile
Cas Number:
17773-41-0
Molecular formula:
C5H9NOS
IUPAC Name:
2-hydroxy-4-(methylsulfanyl)butanenitrile
Details on test material:
See field "Confidential details on test material"
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
not specified
Details on test solutions:
No data available.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
No data available.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
No data available.

Test conditions

Hardness:
No data available.
Test temperature:
No data available.
pH:
No data available.
Dissolved oxygen:
No data available.
Salinity:
Not applicable.
Nominal and measured concentrations:
Nominal concentrations: 16, 22, 32, 48, 70, 100, 140 and 200 ppm (weight/weight) of the initial aqueous solution 10% HMTBN.
Details on test conditions:
TEST SYSTEM
- Test vessel: no data
- No. of organisms per concentration: 20
- No. of organisms per vessel: no data
- No. of vessels per concentration (replicates): no data
- No. of vessels per control (replicates): no data
- No. of vessels per vehicle control (replicates): no data
- Biomass loading rate: no data


TEST MEDIUM / WATER PARAMETERS
No data

EFFECT PARAMETERS MEASURED :
The immobility of the daphnids have been observed at 24 and 48 hours of exposure.


TEST CONCENTRATIONS
No data.

STATISTICAL METHOD:
The CE50-24 and 48 hours and the 95% Confidance Limits have been determined by the probit method.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
87 other: ppm (weight/weight)
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% LC : 74 - 100 ppm (weight/weight)
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
33 other: ppm (weight/weight)
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% LC = 25 - 36 ppm (weight/weight)
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
8.7 other: ppm (weight/weight)
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Remarks:
non diluted HMTBN
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
3.3 other: ppm (weight/weight)
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Remarks:
non diluted HMTBN
Basis for effect:
mobility
Details on results:
The percentage of immobility observed are reported in table 1.

Any other information on results incl. tables

Table 1: Percentage of immobility

   Immobility         
   Number of daphnids / 20     %   
 Concentration in ppm (weight/weight)  24 hours  48 hours  24 hours  48 hours
 Control  0  1  0  5
 200  20  20  100  100
 140  17  20  85  100
 100  8  19  40  95
 70  8  19  40  95
 48  3  11  15  55
 32  1  12  5  60
 22  1  6  5  30
 16  1  1  5  5

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
Based on the results of this study, HMTBN is considered as Toxic to the aquatic organisms tested in accordance with the Directive 67/548/EC.
Executive summary:

The ability of HMTBN to immobilise Daphnia magna was tested in an acute toxicity study according to the EU Directive 92/69/EEC C.2.

The following concentrations were tested : 16, 22, 32, 48, 70, 100, 140 and 200 ppm (weight/weight) of test item (aqueous solution 10% HMTBN). No analytical monitoring has been performed. No other information is available on the test conditions.

There was 5% mortality of daphnia magna in the controls at the end of the exposure period (48h). The EC50(48 h)-value was estimated to be 33 ppm (w/w) with a 95% CL of 25 -36 ppm (w/w) based on the test item. This result correspond to an EC50(48 h)-value of 3.3 ppm (w/w) based on HMTBN non diluted.

Based on the results of this study, HMTBN is considered as Toxic to the aquatic organisms tested in accordance with the Directive 67/548/EC.