Registration Dossier
Registration Dossier
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EC number: 216-700-6 | CAS number: 1643-20-5
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01-03-1990 to 20-03-1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- The quantitative reduction in the proliferation of bacteria caused by the test substance was assessed, measured as a comparison to the control in duplicate. The optical density was used.
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material
No data
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 126 deg C
- Boiling point: n/a
- Vapour pressure: 1.7E-06 and 7.5E-05 Pa
- Water solubility: 409 g/L
- Henry's law constant: 3.7E-09-1.2E-08 Pa m³/mol
- log Pow: <2.7
- pKa: 4.01
- Stability in water: stable
- Stability in light: stable
- pH dependance on stability: n/a
OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: readily biodegradable - Analytical monitoring:
- not specified
- Details on sampling:
- No data
- Vehicle:
- not specified
- Details on test solutions:
- No data
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture.
- Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 18 h
- Post exposure observation period:
- No data
- Hardness:
- No data
- Test temperature:
- No data
- pH:
- No data
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal: 0, 50, 100, 600, 10000 and 100000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL Erlenmeyer flasks
- Type: Closed
- Material, size, headspace, fill volume: 80 mL
- Aeration: Sealed
- No. of vessels per concentration: Duplicate
- No. of vessels per control: Duplicate - Reference substance (positive control):
- no
- Duration:
- 18 h
- Dose descriptor:
- EC10
- Effect conc.:
- 80 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 18 h
- Dose descriptor:
- EC10
- Effect conc.:
- 24 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Details on results:
- No data
- Results with reference substance (positive control):
- No data
- Reported statistics and error estimates:
- No data
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The EC10 of the test item was determined to be 80 mg/L.
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 25-03-1992 to 26-03-1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: German Standard Method DIN 38 412
- Deviations:
- not specified
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 126 deg C
- Boiling point: n/a
- Vapour pressure: 1.7E-06 and 7.5E-05 Pa
- Water solubility: 409 g/L
- Henry's law constant: 3.7E-09-1.2E-08 Pa m³/mol
- log Pow: <2.7
- pKa: 4.01
- Stability in water: stable
- Stability in light: stable
- pH dependance on stability: n/a
OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: readily biodegradable - Analytical monitoring:
- not specified
- Vehicle:
- no
- Test organisms (species):
- Pseudomonas putida
- Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 16 h
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- 76 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- 188.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 16 h
- Dose descriptor:
- EC100
- Effect conc.:
- 588 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- 22.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Validity criteria fulfilled:
- yes
- Remarks:
- the test substance and the contol were in the prescribed ranges.
- Conclusions:
- The toxicity of the test substance was assessed in a cell multiplication inhibition test using Pseudomonas putida according to the German Standard Method DIN 38 412. The EC50 after 16 h was 188.7 mg/L.
Referenceopen allclose all
Bacterial toxicity of test item by the Bringmann-Kuhn method:
Concentration test item (mg/L) |
Cell proliferation Absorbance at 420 nm |
|
Test series 1 |
Test series 2 |
|
0 |
0.185 |
0.187 |
50 |
|
0.155 |
100 |
0.146 |
0.156 |
600 |
|
0.079 |
1000 |
0.056 |
0.056 |
10000 |
0.040 |
|
100000 |
0.020 |
|
Description of key information
No reliable studies are available for C12 AO. Data are read across from C12-14 AO on the basis that this substance contains a significant amount of C12 AO and the remainder comprises an analogue substance with very similar chain length.
Two reliable studies are available for C12 -14 AO. In the key study, Pseudomonas putida were exposed to C12 -14 AO for 18 hours in a Bringmann-Kuehn test. The EC10, based on reduction in growth rate, was 24 mg AO/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 24 mg/L
Additional information
No reliable studies are available for C12 AO. Data are read across from C12-14 AO on the basis that this substance contains a significant amount of C12 AO and the remainder comprises an analogue substance with very similar chain length.
Two reliable studies are available for C12 -14 AO. In the key study, Pseudomonas putida were exposed to nominal concentrations of 0, 50, 100, 600, 1000, 10000 or 100000 mg/L of C12 -14 AO for 18 hours in a Bringmann-Kuehn test [Kolvenbach M (1990)]. The EC10, based on reduction in growth rate, was 24 mg AO/L.
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