Registration Dossier
Registration Dossier
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EC number: 219-207-4 | CAS number: 2386-87-0
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The impact of Celloxide 2021P on the respiration rate of activated sludge was assessed according to the OECD Guideline 209 (Adopted 1984).
Samples of activated sludge were exposed, for three hours, to a range of concentrations of Celloxide 2021P. Each preparation contained dechlorinated tap water and a synthetic sewage preparation that provided a uniform respiration substrate. At the end of the exposure period, the respiration rate of the activated sludge microbes was determined by measuring the decline in oxygen concentration of the test culture. The inhibitory effect of the test substance was calculated by comparing the respiration rate of cultures containing test substance to that of blank control cultures containing only activated sludge, dechlorinated tap water and synthetic sewage. A series of cultures containing a reference substance (3,5‑dichlorophenol) confirmed that the sensitivity of the sludge was within acceptable limits.
In the definitive test, activated sludge was exposed to Celloxide 2021P at nominal concentrations of 19, 61, 195, 625 and 2000 mg/L. The mean respiration rates observed at each of the test concentrations were 71, 71, 68, 57 and 49 mg O2/L/h respectively. The mean respiration rates for the 19, 61 and 195 mg/L test concentrations were within the range observed for the blank control samples (67 to 75 mgO2/L/h) suggesting no inhibitory effect. However, at the 625 and 2000 mg/L test concentrations, an inhibitory effect of 19% and 31% respectively was observed.
Based on Probit analysis, the effective concentrations of Celloxide 2021P that caused a 10% and 20% reduction in respiration rate relative to the untreated controls (EC10and EC20) were 409 and 917 mg/L respectively. The EC50and EC80could not be reliably determined but can be said to be >2000 mg/L. The no observed effect concentration (NOEC) is considered to be 195 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 2 000 mg/L
- EC10 or NOEC for microorganisms:
- 195 mg/L
Additional information
The impact of Celloxide 2021P on the respiration rate of activated sludge was assessed according to the OECD Guideline 209 (Adopted 1984). Based on Probit analysis, the effective concentrations of Celloxide 2021P that caused a 10% and 20% reduction in respiration rate relative to the untreated controls (EC10and EC20) were 409 and 917 mg/L respectively. The EC50and EC80could not be reliably determined but can be said to be >2000 mg/L. The no observed effect concentration (NOEC) is considered to be 195 mg/L.
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