Trideca-1,1,1,2,2,3,3,4,4,5,5,6,6-fluorohexane

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994-01-31 to 1994-02-02

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Japanese Industrial Standard (JIS K 0102-1986-71.), 'Testing methods for industrial waste water, Acute toxicity test with fish.

Deviations:

not specified

Principles of method if other than guideline:

The test was performed in accordance with Japanese Industrial Standard (JIS K 0102-1986-71.), 'Testing methods for industrial waste water, Acute toxicity test with fish.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Asahi Glass Co., Ltd. (Japan); Batch no. 930817
- Expiration date of the lot/batch: not specified
- Purity test date: 1993-12-20 (before initiation of exposure)

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: cold dark storage place
- Stability under test conditions: stable during exposure period

FORM AS APPLIED IN THE TEST (if different from that of starting material): Colourless and Transparent Liquid

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 125 mg/L
- Sampling method: Test substance and Dispersant (MEGAFAC F-142D; 10 times amount of the test substance) were mixed. The mixture was then diluted with ion-exchanged water to prepare 1000 mg/L stock solution.
- Sample storage conditions before analysis: Stock solution refrigerated and supplied to test tank

Vehicle:

yes

Remarks:

ion-exchanged water

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance and Dispersant (MEGAFAC F-142D; 10 times amount of the test substance) were mixed. The mixture was then diluted with ion-exchanged water to prepare 1000 mg/L stock solution.
- Controls: not specified
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Dispersant: MEGAFAC F-142D

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Orange-red killifish
- Strain: not specified
- Source: Nakshima Fish Farm (Japan)
- Age at study initiation (mean and range, SD): Not specified
- Length at study initiation (length definition, mean, range and SD): Average length: 2.9 cm
- Weight at study initiation (mean and range, SD): Average weight: 0.22 grams
- Method of breeding: not specified
- Maintenance of the brood fish: Fish were visually examined upon receipt and those demonstrating any abnormalities were removed. The fish were reared for 3 days in a flow through system following an external disinfection.

ACCLIMATION
- Acclimation period: After rearing, fish were transferred to an acclimatiing aquarium and acclimatized there after the second external disinfection. Fish demonstrating any abnormalities were removed and reared for 12 days in a flow through system at a temperature of 25 ± 2°C. Fish were then checked for health conditions and reared for another 39 days after the external disinfection.
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: not specified
- Feeding frequency during acclimation: not specified
- Health during acclimation (any mortality observed): not specified

Test type:

semi-static

Water media type:

other: Underground water from the premises of Kurume Research Laboratories

Total exposure duration:

48 h

Test temperature:

25 ± 2°C

pH:

At initial exposure = 7.9
At termination of exposure = 7.3

Dissolved oxygen:

At initial exposure = 7.8 mg/L
At termination of exposure = 6.0 mg/L

Nominal and measured concentrations:

125 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass Gallon Bottle
- Type (delete if not applicable): open / closed (not specified)
- Renewal rate of test solution (frequency/flow rate): every 8-16 hours
- No. of organisms per vessel: 10/level

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Underground water from the premises of Kurume Research Laboratories

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 125 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Cumulative Mortality
Concentration (mg/L)	Cumulative Mortality (%)
	2 hours	24 hours	48 hours
Control	-	0	0
125	-	0	0

Validity criteria fulfilled:

not specified

Conclusions:

The 48-hour LC50 of the test material in Oryzias latipes was >125 mg/L.

Executive summary:

The 48-hour LC₅₀ of the test material in Oryzias latipes was >125 mg/L.

Description of key information

The 48-hour LC₅₀ of 1,1,1,2,2,3,3,4,4,5,5,6,6-tridecafluorohexane in Oryzias latipes was determined to be >125 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

125 mg/L

Additional information

In a key short-term toxicity study in fish (Kurume Research Laboratories, 1994b), conducted in accordance with Japanese Industrial Standard (JIS K 0102-1986-71), Orange-red killifish (Oryzias latipes) were exposed to 1,1,1,2,2,3,3,4,4,5,5,6,6-tridecafluorohexane at concentrations of 125 mg/L (mixed with dispersant (MEGAFAC F-142D) and diluted with ion-exchanged water) for a period off 48 hours.

 

The 48-hour LC₅₀ of 1,1,1,2,2,3,3,4,4,5,5,6,6-tridecafluorohexane in Oryzias latipes was determined to be >125 mg/L.