Reaction mass of 2-Piperazin-1-ylethanol and Piperazine-1,4-diethanol and Piperazine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable study report which meets basic scientific principles. Hydroxyethylpiperazine is the major constituent (ca. 40%) of the test substance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1967

Report date:

1967

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Version / remarks:

(adopted on 1981, 12th may; inhalation hazard test)

Deviations:

yes

Remarks:

(animals were observed for only 7 days, only 3 animals for each exposure time were used, no details only animal husbandry, exposure period of 8 hours, concentration of test substance in air mixture was not determined)

GLP compliance:

no

Test type:

other: inhalation hazard test (IHT)

Test material

Test material information

Constituent 1

Reference substance name:

2-piperazin-1-ylethanol

EC Number:

203-142-3

EC Name:

2-piperazin-1-ylethanol

Cas Number:

103-76-4

Molecular formula:

C6H14N2O

IUPAC Name:

2-piperazin-1-ylethanol

Details on test material:

- Name of test material (as cited in study report): Hydroxyaethylpiperazin
- Analytical purity: 99 %

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 172 g (mean)

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

not specified

Vehicle:

air

Details on inhalation exposure:

The test demonstrates the toxicity of an atmosphere saturated with vapours of the volatile components of the test substance at the temperature chosen for vapour generation (20°C). 3 rats per sex were exposed to the vapours, generated by bubbling 200 L/h air through a substance column of about 5 cm above a fritted glassdisc in a glass cylinder for 8h. The documentation of clinical signs was performed over a period of 7 days. In order to verify the results, the test was repeated once with new groups of animals.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

8 h

Concentrations:

In the study report and the raw data no substance loss but an increase in substance weight was recorded.

No. of animals per sex per dose:

3

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

other: Inhalation Risk Test

Based on:

test mat.

Exp. duration:

8 h

Remarks on result:

other: No mortality occurred.

Mortality:

No mortality occurred.

Clinical signs:

other: No clinical signs observed.

Body weight:

The treated animals gained weight (mean weight at study end: 199 g).

Gross pathology:

In one animal, chronic bronchitis and bronchiectasis in the right superior lobe of the lung were observed. Other animals were negative.