4-amino-5-methoxy-N,2-dimethylbenzenesulphonamide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well performed OECD study with GLP but without anaytical determination of the test substance

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-amino-5-methoxy-N,2-dimethylbenzenesulphonamide

EC Number:

256-380-5

EC Name:

4-amino-5-methoxy-N,2-dimethylbenzenesulphonamide

Cas Number:

49564-57-0

Molecular formula:

C9H14N2O3S

IUPAC Name:

4-amino-5-methoxy-N,2-dimethylbenzene-1-sulfonamide

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The test item was homogenized by an Ultra-Turrax and transferred into the bassin. Then, the content of the bassin was strirred for two hours. The test item was as well solved in water as also found as a precipitate on the bottom ogf the bassin. The concentration given is not the solved part of the test item but is the total amount of substance addid into the bassin.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrabärbling, Brachydanio rerio
- Strain: Hamilton-Buchanan
- Source: West Aquarium; 3422 Bad Lauterberg
- Length at study initiation : 2.5 - 3.1 cm
- Feeding during test: twice a day, ad libitum
- Food type: Tetra Min, Tetra Werke, Melle

ACCLIMATION
- Acclimation period: 14 days

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

21.3 - 23.0 °C

pH:

7.0 - 8.0

Dissolved oxygen:

7.0 - 9.2 mg/L O2

Nominal and measured concentrations:

Nominal: 0, 100, 250, 350, 500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: open glass vessel, 30cm x 22 cm x 24 cm, fill volume 10 liters, no aeration
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

311 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

Based on the results the LC 50 value was determined by the Probitanalyse (method Lindner and Weber, confidence intervall by Fieller)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The LC50 for isabove 100 mg/L. Therefore no classification for toxicity to fish is required.

Executive summary:

Based on the test results and on the Probit-Analyse the LC50 of the test item was determined as 311 mg/L(222 -422mg/L) after 96 hours.