Manganese, 4-[(5-chloro-4-methyl-2-sulfophenyl)azo]-3-hydroxy-2-naphthalenecarboxylic acid complex

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: the test was performed with an aqueous extract (eluate) of the test substance. The nominal concentration of the eluate was 100 mg/L. The test substance was stirred in M4 medium for about 20 hours at 20 ± 2 °C. Undissolved test substance was removed by centrifugation (20 minutes at 17700G)
- Eluate: yes

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Daphnia magna
- Source: the clone of Daphnia magna used was supplied by Institut National de Recherche Chimique Appliquée, France, in 1978. The daphnids are cultured under standard conditions in the laboratory of ecotoxicology, BASF AG, Ludwigshafen".
- Age at study initiation: 2-24 h
- Feeding during test: No

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.42 mmol/L

Test temperature:

20.3 - 20.5 °C

pH:

8.0 - 8.1

Dissolved oxygen:

8.4 - 8.7

Nominal and measured concentrations:

Nominal concentrations: Control, 12.5, 25, 50, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Test tubes (glass) with fIat bottom (nominal volume 20 mL)
- Fill volume: 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic medium M4

This synthetic medium has the following properties:
- Total hardness 2.20 - 3.20 mmol/L
- Alkalinity up to pH 4.3: 0.80 - 100 mmol/L
- Molar ratio Ca/Mg: about 4:1
- pH value: 7.5-8.5
- Conductivity : 550 - 650 µS/cm

OTHER TEST CONDITIONS
- Photoperiod: Artificial Iight, OSRAM L58 W31 warm white; day/night-rhythm = 16 / 8 hours
- Light intensity: About 5 - 6 µE/(m2xS) in the range of 400 - 700 nm


EFFECT PARAMETERS MEASURED
- Swimming ability of the test animals: visually after 0, 24 and 48 h

Reference substance (positive control):

yes

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: no effects observed up to the limit of water solubility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No acute toxicity within the range of solubility.

Results with reference substance (positive control):

The 24h-EC50 of the control substance potassium dichromate was 1,29 mg/L. Date of the last control experiment: 23 Oct 2000, project-no: 97/0242/50/50

The test substance showed no inhibitory effect in the range of its water solubility.

Validity criteria fulfilled:

yes

Conclusions:

With high probability acutely not harmful to aquatic invertebrates.

Description of key information

With high probability acutely not harmful to aquatic invertebrates.

Additional information

A GLP-guideline study according to OECD 202 was conducted using Daphnia magna (BASF AG, 2000). At test termination no effects were observed up to the limit of water solubility (at a nominal loading rate of 100 mg/L).