4-pentylcyclohexanone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May - August 1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Details on sampling:

An analytical control measurement of the actual concentration of the test substance in the different test batches was not done, because the highest concentration (0.1 g/L) soluble in water which was tested is below the water solubility. There was no reason to assume the the concentration in the test vessels should differ form the nominal concentration.

Vehicle:

no

Details on test solutions:

The individual concentrations of the test substance in the different test batches resulted from weighing in calculated amounts of the test substance directly into the given volume of water in the test chambers.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium GmbH + Co. KG, D- 3422 Bad Lauterberg (Art. No. 770874)
- Age at study initiation: about 2 month
- Length at study initiation: average total length 2.0 ± 1.0 cm

ACCLIMATION
- Acclimation period: 13/10/1988 till 30/04/1990
- Acclimation conditions: same as test conditions
- Type and amount of food during acclimation: breeding food for carps (Club-Kronen Tiernahrung GmbH & Co. KG, Wesel).
- Health during acclimation: no mortality observed

FEEDING DURING TEST
no feeding during test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

19.1 - 19.6 °C

pH:

7.7 - 8.2

Dissolved oxygen:

97 - 100 %

Nominal and measured concentrations:

Nominal concentrations: 1, 1.8, 3.2, 5.6, 10, 100 mg/L
no measured concentrations

Details on test conditions:

TEST SYSTEM
- Test vessel: glass tanks
- Type: closed with fitted glass plates
- Material, size: glass, length 300 mm, width 200 mm, height 220 mm
- Aeration: yes, continuous
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: Reconstituted water according to OECD 203

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: no details available
- Light intensity: no details available

EFFECT PARAMETERS MEASURED
At 0, 1, 24, 48, 72 and 96 h after the start of the test, the fish of each test batch were inspected for mortality.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

7.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

5.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The validation criteria according to OECD 203 were fullfiled: Dissolved oxygen concentration was not less than 60 % of the air saturation value, the pH wasin the range between 6.0 and 8.5 and the temperature of the test medium was between 21 and 25°C.

Reported statistics and error estimates:

The percentage of mortality at each concentration between LC-0 and LC-100 was transformed into a particular Probit-value. Since there is no Probit-value for 0 % and 100 % effect and - on the other hand - the values at the edges of the toxicity-range are regarded valuable for the evaluation of the results, the Probit for 0 % was said to be 3.36 (this corresponds to 5 %) and the Probit for 100 % was said to be 6.65 (this corresponds to 95 %). These values were chosen, because a result of 0 (or 10) dead fish can mean any percentage between 0 % and 100 % (or between 90'% and 100 % on the upper edge), since there were only 10 - not 100 - fish tested. The mean of this inaccuracy is 5 % (or 95 %, respectively). With these values, a linear regression analysis of the Probit = f(log c) function was performed.

Sublethal observations / clinical signs:

 Table 1: Cumulative mortality at intervals during the test, cumulative percentage mortality in ( ):

Concentration [mg/L]	time after test start [h]
	0 (0)	24	48	72	96
0	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)
1	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)
1.8	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)
3.2	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)
5.6	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)
10	0 (0)	5 (50)	10 (100)	10 (100)	10 (100)
100	0 (0)	10 (100)	10 (100)	10 (100)	10 (100)

 

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity test with Zebrafish was performed according to OECD 203. Under the given conditions the 96h LC50 value was 7.5 mg/L.

Executive summary:

An investigation of the lethal effects of test substance to the Zebra-fish (Brachydanio rerio) was carried out according to OECD Guideline 203 "Fish, Acute Toxicity Test". Fish were exposed in separate test batches to different concentrations of test substance in the test medium over a test period of 96 h (each tank with 10 fish). The concentrations used in the test were 0, 1, 1.8, 3.2, 5.6, 10 and 100 mg/L. Criteria for the inspections of test fish during the exposure period were, above all, the mortality rates, but moreover abnormal symptoms in the swimming behaviour, in visible physiological functions as well as in the outward appearance of the test fish were recorded. Fish were considered dead if touching of the caudal peduncle produced no reaction and if no breathing movements could be observed. The toxic properties of the test substance were found to be as follows: LC100 = 10 mg/L, LC50 = 7.5 mg/L, NOEC = 5.6 mg/L.

Description of key information

The acute toxicity test with Zebrafish was performed according to OECD 203. Under the given conditions the 96h LC50 value was 7.5 mg/L (reference 6.1.1-1).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

7.5 mg/L

Additional information

An investigation of the lethal effects of test substance to the Zebra-fish (Brachydanio rerio) was carried out according to OECD Guideline 203 "Fish, Acute Toxicity Test". Fish were exposed in separate test batches to different concentrations of test substance in the test medium over a test period of 96 h (each tank with 10 fish). The concentrations used in the test were 0, 1, 1.8, 3.2, 5.6, 10 and 100 mg/L. Criteria for the inspections of test fish during the exposure period were, above all, the mortality rates, but moreover abnormal symptoms in the swimming behaviour, in visible physiological functions as well as in the outward appearance of the test fish were recorded. Fish were considered dead if touching of the caudal peduncle produced no reaction and if no breathing movements could be observed. The toxic properties of the test substance were found to be as follows: LC100 = 10 mg/L, LC50 = 7.5 mg/L, NOEC = 5.6 mg/L (reference 6.1.1 -1).