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EC number: 473-370-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 October 2007 to 28 November 2007
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
- Principles of method if other than guideline:
- the exposure duration was 48 hours, while the guidelne prefers a 72 hour exposure
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/L (vessel without algae), 0,10, 18, 32, 56 and 100 mg/L at t=0, t=24 and t=48 h
- Sampling method: 1.5 mL samples
- Sample storage conditions before analysis: in freezer - Vehicle:
- yes
- Remarks:
- water
- Details on test solutions:
- Solutions were ultrasonicated for 10 minutes followed by magnetic stirring during 18 minutes. The solutions were clear and colorless
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: NIVA CL1
- Source: in-house laboratory culture
- Age of inoculum (at test initiation): pre-cultured for 3 days
- Method of cultivation: cutured in M1 medium; pre-culture and test in M2 medium (AAP according to the OECD guideline)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- exposure duration deviates from the preferred duration of 72 hours
- Hardness:
- 24 mg/L as CaCO3
- Test temperature:
- 21.9-23.7 ˚C
- pH:
- 7.9-8.2
- Nominal and measured concentrations:
- nominal 0,10, 18, 32, 56 and 100mg/L
measured concentrations were 98-109% of nominal. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL, glass
- Type: open
- Fill volume: 50 mL
- Initial cells density: 1.0E04 cells/mL
- Control end cells density: 55.8E04 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AAP medium according to OECD 201
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: continuous light
- Light intensity and quality: TLD lamps 86-92 µE.m-2.s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: initial counting chamber (under microscope); thereafter at t=24 and t=48 h with spectrophotometer at 720 nm
- Chlorophyll measurement: none
- Other: any abnormal appearance of the algae at test end
TEST CONCENTRATIONS
- Range finding study: at control, 0.1,1.0,10 and 100 mg/L during 48 hours
- Results used to determine the conditions for the definitive study: cell count at 48 h: 53.4, 52.4, 50.6, 48.7 and 1.4* E04 cells/L at control, 0.1,1.0,10 and 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate at 0.18 to 3.2 mg/L during 72 hours
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 58 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 30 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: see remarks
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- At 10 and 18 mg/L growth rate reduction was <10% over the 48 h study period. This could be considered a biologically irrelevant, But as during the first 24 hous the growth rate reduction was 1.6-3.6% and during the second 24 hours 8.1-10.5%, it can be assumed that when extending the test up to 72 hours would lead a growth rate decrease > 10%. Therefore the value of the NOEC is set at <10 mg/L.
A similar increase of growth rate reduction with time was observed at all tested concentrations - Results with reference substance (positive control):
- EC50 growth rate 2.1 mg/L
EC50 biomass 0.85 mg/L - Reported statistics and error estimates:
- All reported reduction of growth rate and inhibition of biomass were statistically significant in at least one of the statistical methods applied (Bonferroni t-test, Tuckey method)
mean coefficient of variation for section-by-section specific growth rates in the control cultures was < 35% (ANOVA) - Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-h EC50 for growth rate is 58 mg/L
The NOEC for growth rate is < 10 mg/L - Executive summary:
In a test according to OECD 201, green algae were exposed during 48 hours to 0, 10, 18, 32, 56 and 100 mg/L of the test substance. There were statistically significant effects on growth rate and biomass for all tested concentrations. Reduction of growth rate was shown to increase with increasing study duration.
The EC50 for growth rate is 58 mg/L and for biomass 30 mg/L. The NOEC is < 10 mg/L.
Reference
Description of key information
In a test according to OECD 201, green algae were exposed during 48 hours to 0, 10, 18, 32, 56 and 100 mg/L of the test substance. There were statistically significant effects on growth rate and biomass for all tested concentrations. Reduction of growth rate was shown to increase with increasing study duration.
The EC50 for growth rate is 58 mg/L and for biomass 30 mg/L. The NOEC is < 10 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 58 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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